Signal Transduction Antibodies 2
Anti-AQP2 Antibody (CAB16209)
- SKU:
- CAB16209
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-AQP2 Antibody |
| Antibody SKU: | CAB16209 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, mouse kidney, rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 147-271 of human AQP2 (NP_000477.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ASTD ERRG ENPG TPAL SIGF SVAL GHLL GIHY TGCS MNPA RSLA PAVV TGKF DDHW VFWI GPLV GAIL GSLL YNYV LFPP AKSL SERL AVLK GLEP DTDW EERE VRRR QSVE LHSP QSLP RGTK A |
| Gene ID: | 359 |
| Uniprot: | P41181 |
| Cellular Location: | Apical cell membrane, Basolateral cell membrane, Cytoplasmic vesicle membrane, Golgi apparatus, Multi-pass membrane protein, trans-Golgi network membrane |
| Calculated MW: | 28kDa |
| Observed MW: | 29kDa |
| Synonyms: | AQP2, AQP-CD, WCH-CD |
| Background: | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. |
| UniProt Protein Function: | AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| UniProt Protein Details: | Protein type:Transporter, aquaporin family; Membrane protein, integral; Channel, misc.; Transporter; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12q12-q13 Cellular Component: recycling endosome; protein complex; clathrin-coated vesicle; membrane; rough endoplasmic reticulum; integral to plasma membrane; lysosome; basolateral plasma membrane; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network Molecular Function:water transporter activity; glycerol transmembrane transporter activity; actin binding; water channel activity; PDZ domain binding; glycerol channel activity Biological Process: glycerol transport; actin filament depolymerization; cellular water homeostasis; response to glucagon stimulus; apoptosis; water transport; response to lithium ion; cellular response to water deprivation; response to lipopolysaccharide; female pregnancy; excretion; response to salt stress; response to starvation; hyperosmotic response; renal water transport; renal water homeostasis; response to calcium ion; positive regulation of calcium ion transport; transmembrane transport; aging Disease: Diabetes Insipidus, Nephrogenic, Autosomal |
| NCBI Summary: | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P41181 |
| NCBI GenInfo Identifier: | 728874 |
| NCBI Gene ID: | 359 |
| NCBI Accession: | P41181.1 |
| UniProt Related Accession: | P41181 |
| Molecular Weight: | |
| NCBI Full Name: | Aquaporin-2 |
| NCBI Synonym Full Names: | aquaporin 2 |
| NCBI Official Symbol: | AQP2 |
| NCBI Official Synonym Symbols: | AQP-CD; WCH-CD |
| NCBI Protein Information: | aquaporin-2 |
| UniProt Protein Name: | Aquaporin-2 |
| UniProt Synonym Protein Names: | ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct |
| UniProt Gene Name: | AQP2 |
| UniProt Entry Name: | AQP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AQP2 antibody (CAB16209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 antibody (CAB16209) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat kidney cells using AQP2 antibody (CAB16209) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse kidney cells using AQP2 antibody (CAB16209) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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