Immunology Antibodies 2
Anti-APOBEC3F Antibody (CAB17269)
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- SKU:
- CAB17269
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-APOBEC3F Antibody |
| Antibody SKU: | CAB17269 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 189-373 of human APOBEC3F (NP_660341.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | PC-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 189-373 of human APOBEC3F (NP_660341.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RNPM EAMY PHIF YFHF KNLR KAYG RNES WLCF TMEV VKHH SPVS WKRG VFRN QVDP ETHC HAER CFLS WFCD DILS PNTN YEVT WYTS WSPC PECA GEVA EFLA RHSN VNLT IFTA RLYY FWDT DYQE GLRS LSQE GASV EIMG YKDF KYCW ENFV YNDD EPFK PWKG LKYN FLFL DSKL QEIL E |
| Gene ID: | 200316 |
| Uniprot: | Q8IUX4 |
| Cellular Location: | |
| Calculated MW: | 9kDa/11kDa/45kDa |
| Observed MW: | 45kDa |
| Synonyms: | APOBEC3F, A3F, ARP8, BK150C2.4.MRNA, KA6 |
| Background: | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA processing; Hydrolase; EC 3.5.4.- Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; ribonucleoprotein complex Molecular Function:protein binding; zinc ion binding; RNA binding; cytidine deaminase activity Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus |
| NCBI Summary: | This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8IUX4 |
| NCBI GenInfo Identifier: | 161784334 |
| NCBI Gene ID: | 200316 |
| NCBI Accession: | Q8IUX4.3 |
| UniProt Secondary Accession: | Q8IUX4,Q45F03, Q6ICH3, Q7Z2N2, Q7Z2N5, B0QYD4, |
| UniProt Related Accession: | Q8IUX4 |
| Molecular Weight: | 373 |
| NCBI Full Name: | DNA dC-> |
| NCBI Synonym Full Names: | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F |
| NCBI Official Symbol: | APOBEC3F |
| NCBI Official Synonym Symbols: | A3F; KA6; ARP8; BK150C2.4.MRNA |
| NCBI Protein Information: | DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3F; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like |
| UniProt Protein Name: | DNA dC->dU-editing enzyme APOBEC-3F |
| UniProt Synonym Protein Names: | Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F |
| Protein Family: | DNA dC->dU-editing enzyme |
| UniProt Gene Name: | APOBEC3F |
| UniProt Entry Name: | ABC3F_HUMAN |
 | Western blot analysis of extracts of PC3 cells, using APOBEC3F antibody (CAB17269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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