Cell Biology Antibodies 4
Anti-APOA5 Antibody (CAB1424)
- SKU:
- CAB1424
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-APOA5 Antibody |
| Antibody SKU: | CAB1424 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 207-366 of human APOA5 (NP_443200.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 207-366 of human APOA5 (NP_443200.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QELH RSVA PHAP ASPA RLSR CVQV LSRK LTLK AKAL HARI QQNL DQLR EELS RAFA GTGT EEGA GPDP QMLS EEVR QRLQ AFRQ DTYL QIAA FTRA IDQE TEEV QQQL APPP PGHS AFAP EFQQ TDSG KVLS KLQA RLDD LWED ITHS LHDQ GHSH LGDP |
| Gene ID: | 116519 |
| Uniprot: | Q6Q788 |
| Cellular Location: | Secreted |
| Calculated MW: | 41kDa |
| Observed MW: | 41KDa |
| Synonyms: | APOA5, APOAV, RAP3 |
| Background: | The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. |
| UniProt Protein Function: | APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Lipid-binding Chromosomal Location of Human Ortholog: 11q23 Cellular Component: chylomicron; extracellular region; extracellular space Molecular Function:cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding Biological Process: acylglycerol homeostasis; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; positive regulation of receptor-mediated endocytosis; regulation of cholesterol absorption; reverse cholesterol transport; tissue regeneration; triacylglycerol catabolic process; triacylglycerol metabolic process Disease: Hyperlipoproteinemia, Type V; Hypertriglyceridemia, Familial |
| NCBI Summary: | The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q6Q788 |
| NCBI GenInfo Identifier: | 60391728 |
| NCBI Gene ID: | 116519 |
| NCBI Accession: | Q6Q788.1 |
| UniProt Secondary Accession: | Q6Q788,Q3MIK6, Q6UWK9, Q9UBJ3, B0YIV9, |
| UniProt Related Accession: | Q6Q788 |
| Molecular Weight: | 41,213 Da |
| NCBI Full Name: | Apolipoprotein A-V |
| NCBI Synonym Full Names: | apolipoprotein A5 |
| NCBI Official Symbol: | APOA5 |
| NCBI Official Synonym Symbols: | RAP3; APOAV |
| NCBI Protein Information: | apolipoprotein A-V |
| UniProt Protein Name: | Apolipoprotein A-V |
| UniProt Synonym Protein Names: | Apolipoprotein A5; Regeneration-associated protein 3 |
| Protein Family: | Apolipoprotein |
| UniProt Gene Name: | APOA5 |
| UniProt Entry Name: | APOA5_HUMAN |
 | Western blot analysis of extracts of Mouse liver, using APOA5 antibody (CAB1424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00032). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human liver using APOA5 antibody (CAB1424) at dilution of 1:100 (40x lens). |
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