Immunology Antibodies 1
Anti-APOA2 Antibody (CAB14690)
- SKU:
- CAB14690
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-APOA2 Antibody |
| Antibody SKU: | CAB14690 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human APOA2 (NP_001634.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Human plasma |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human APOA2 (NP_001634.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKLL AATV LLLT ICSL EGAL VRRQ AKEP CVES LVSQ YFQT VTDY GKDL MEKV KSPE LQAE AKSY FEKS KEQL TPLI KKAG TELV NFLS YFVE LGTQ PATQ |
| Gene ID: | 336 |
| Uniprot: | P02652 |
| Cellular Location: | Secreted |
| Calculated MW: | 11kDa |
| Observed MW: | 11kDa |
| Synonyms: | APOA2, Apo-AII, ApoA-II, apoAII |
| Background: | This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. |
| UniProt Protein Function: | Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. |
| UniProt Protein Details: | Subunit structure: Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP. Ref.14 Ref.15 Ref.18 Subcellular location: Secreted. Tissue specificity: Plasma; synthesized in the liver and intestine. Post-translational modification: Phosphorylation sites are present in the extracelllular medium. Sequence similarities: Belongs to the apolipoprotein A2 family. Mass spectrometry: Molecular mass is 8701.2 Da from positions 24 - 100. Determined by MALDI. Ref.16Molecular mass is 8823.4 Da from positions 24 - 100. Determined by MALDI. Cysteinylated ApoA-II. Ref.16Molecular mass is 17421.3 Da from positions 24 - 100. Determined by MALDI. Homodimer. Ref.16Molecular mass is 17293.4 Da from positions 24 - 100. Determined by MALDI. Heterodimer with apolipoprotein A-II(1-76). Ref.16Molecular mass is 8578.3 Da from positions 24 - 99. Determined by MALDI. Ref.16Molecular mass is 17166.2 Da from positions 24 - 99. Determined by MALDI. Homodimer. Ref.16 |
| NCBI Summary: | This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq] |
| UniProt Code: | P02652 |
| NCBI GenInfo Identifier: | 114000 |
| NCBI Gene ID: | 336 |
| NCBI Accession: | P02652.1 |
| UniProt Secondary Accession: | P02652,B2R524, |
| UniProt Related Accession: | P02652,Q76EI7 |
| Molecular Weight: | 11,175 Da |
| NCBI Full Name: | Apolipoprotein A-II |
| NCBI Synonym Full Names: | apolipoprotein A-II |
| NCBI Official Symbol: | APOA2 |
| NCBI Official Synonym Symbols: | apoAII; Apo-AII; ApoA-II |
| NCBI Protein Information: | apolipoprotein A-II; apolipoprotein A2; OTTHUMP00000032244 |
| UniProt Protein Name: | Apolipoprotein A-II |
| UniProt Synonym Protein Names: | Apolipoprotein A2 |
| Protein Family: | Apolipoprotein |
| UniProt Gene Name: | APOA2 |
| UniProt Entry Name: | APOA2_HUMAN |
 | Western blot analysis of extracts of human plasma, using APOA2 antibody (CAB14690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using APOA2 Rabbit pAb (CAB14690) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using APOA2 Rabbit pAb (CAB14690) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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