KO Validated Antibodies 1

Anti-APEX1 Antibody (CAB2587)[KO Validated]

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SKU:
CAB2587
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-APEX1 Antibody [KO Validated]
Antibody SKU:CAB2587
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPKR GKKG AVAE DGDE LRTE PEAK KSKT AAKK NDKE AAGE GPAL YEDP PDQK TSPS GKPA TLKI CSWN VDGL RAWI KKKG LDWV KEEA PDIL CLQE TKCS ENKL PAEL QELP GLSH QYWS APSD KEGY SGVG LLSR QCPL KVSY GIGD EEHD QEGR VIVA EFDS FVLV TAYV PNAG RGLV RLEY RQRW DEAF RKFL KGLA SRKP LVLC GDLN VAHE EIDL RNPK GNKK NAGF TPQE RQGF GELL QAVP LADS FRHL YPNT PYAY TFWT YMMN ARSK NVGW RLDY FLLS HSLL PALC DSKI RSKA LGSD HCPI TLYL AL
Gene ID:328
Uniprot:P27695
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Nucleus speckle, nucleolus
Calculated MW:35kDa
Observed MW:39kDa
Synonyms:APEX1, APE, APE1, APEN, APEX, APX, HAP1, REF1
Background:Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
UniProt Protein Function:APE1: a multifunctional enzyme that plays a central role in the cellular response to oxidative stress including DNA repair and redox regulation of transcriptional factors. Binds DNA and RNA. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway, a 3'-5' exoribonuclease for mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules, and a DNA 3' phosphodiesterase capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. Is a loading factor for POLB onto non-incised AP sites in DNA, stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB, and involved in the DNA cleavage step of class switch recombination (CSR). Possesses reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Binds to negative calcium response elements (nCaREs). Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Is an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover. In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Interacts with SIRT1; the interaction is increased in the context of genotoxic stress. Interacts with HDAC1, HDAC2 and HDAC3; the interactions are not dependent on the APEX1 acetylation status. Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate. NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites. Belongs to the DNA repair enzymes AP/ExoA family.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Deoxyribonuclease; Hydrolase; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Lyase; Nucleolus; EC 4.2.99.18; DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: centrosome; cytoplasm; endoplasmic reticulum; mitochondrion; nuclear chromosome, telomeric region; nuclear speck; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm; ribosome

Molecular Function:3'-5' exonuclease activity; chromatin DNA binding; damaged DNA binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; double-stranded DNA specific exodeoxyribonuclease activity; double-stranded telomeric DNA binding; endodeoxyribonuclease activity; endonuclease activity; metal ion binding; oxidoreductase activity; phosphodiesterase I activity; phosphoric diester hydrolase activity; protein binding; ribonuclease H activity; site-specific endodeoxyribonuclease activity, specific for altered base; transcription coactivator activity; transcription corepressor activity; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; base-excision repair, base-free sugar-phosphate removal; DNA repair; positive regulation of DNA repair; regulation of mRNA stability; telomere maintenance

NCBI Summary:Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:P27695
NCBI GenInfo Identifier:113984
NCBI Gene ID:328
NCBI Accession:P27695.2
UniProt Secondary Accession:P27695,Q969L5, Q99775,
UniProt Related Accession:P27695
Molecular Weight:35,555 Da
NCBI Full Name:DNA-(apurinic or apyrimidinic site) lyase
NCBI Synonym Full Names:apurinic/apyrimidinic endodeoxyribonuclease 1
NCBI Official Symbol:APEX1  
NCBI Official Synonym Symbols:APE; APX; APE1; APEN; APEX; HAP1; REF1  
NCBI Protein Information:DNA-(apurinic or apyrimidinic site) lyase
UniProt Protein Name:DNA-(apurinic or apyrimidinic site) lyase
UniProt Synonym Protein Names:APEX nuclease; APEN
Protein Family:DNA-(apurinic or apyrimidinic site) lyase
UniProt Gene Name:APEX1  
UniProt Entry Name:APEX1_HUMAN
Anti-APEX1 Antibody [KO Validated] (CAB2587)Western blot analysis of extracts of various cell lines, using APEX1 antibody (CAB2587). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-APEX1 Antibody [KO Validated] (CAB2587)Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (CAB2587) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-APEX1 Antibody [KO Validated] (CAB2587)Immunofluorescence analysis of HeLa cells using APEX1 antibody (CAB2587). Blue: DAPI for nuclear staining.
Anti-APEX1 Antibody [KO Validated] (CAB2587)Immunofluorescence analysis of A549 cells using APEX1 antibody (CAB2587).
Anti-APEX1 Antibody [KO Validated] (CAB2587)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (CAB2587). Western blot was performed from the immunoprecipitate using APEX1 antibody (CAB2587) at a dilition of 1:1000.
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