Metabolism Antibodies 2

Anti-APC Antibody (CAB2818)

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SKU:
CAB2818
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-APC Antibody
Antibody SKU:CAB2818
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2544-2843 of human APC (NP_000029.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2544-2843 of human APC (NP_000029.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SGTW KREH SKHS SSLP RVST WRRT GSSS SILS ASSE SSEK AKSE DEKH VNSI SGTK QSKE NQVS AKGT WRKI KENE FSPT NSTS QTVS SGAT NGAE SKTL IYQM APAV SKTE DVWV RIED CPIN NPRS GRSP TGNT PPVI DSVS EKAN PNIK DSKD NQAK QNVG NGSV PMRT VGLE NRLN SFIQ VDAP DQKG TEIK PGQN NPVP VSET NESS IVER TPFS SSSS SKHS SPSG TVAA RVTP FNYN PSPR KSSA DSTS ARPS QIPT PVNN NTKK RDSK TDST ESSG TQSP KRHS GSYL VTSV
Gene ID:324
Uniprot:P25054
Cellular Location:Cell junction, Cell membrane, Cell projection, Cytoplasm, adherens junction, cytoskeleton, lamellipodium, ruffle membrane
Calculated MW:300kDa/311kDa
Observed MW:290kDa
Synonyms:APC, BTPS2, DP2, DP2.5, DP3, GS, PPP1R46
Background:This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
UniProt Code:P25054
NCBI GenInfo Identifier:97535708
NCBI Gene ID:324
NCBI Accession:P25054.2
UniProt Secondary Accession:P25054,Q15162, Q15163, Q93042, D3DT03,
UniProt Related Accession:P25054
Molecular Weight:461
NCBI Full Name:Adenomatous polyposis coli protein
NCBI Synonym Full Names:adenomatous polyposis coli
NCBI Official Symbol:APC  
NCBI Official Synonym Symbols:GS; DP2; DP3; BTPS2; DP2.5; PPP1R46  
NCBI Protein Information:adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
UniProt Protein Name:Adenomatous polyposis coli protein
UniProt Synonym Protein Names:Deleted in polyposis 2.5
Protein Family:APC-related protein
UniProt Gene Name:APC  
UniProt Entry Name:APC_HUMAN
Anti-APC Antibody (CAB2818)Western blot analysis of extracts of HeLa cells, using APC antibody (CAB2818) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-APC Antibody (CAB2818)Immunohistochemistry of paraffin-embedded human lung using APC antibody (CAB2818) at dilution of 1:200 (40x lens).
Anti-APC Antibody (CAB2818)Immunohistochemistry of paraffin-embedded human colon using APC antibody (CAB2818) at dilution of 1:200 (40x lens).
Anti-APC Antibody (CAB2818)Immunohistochemistry of paraffin-embedded human breast cancer using APC antibody (CAB2818) at dilution of 1:200 (40x lens).
Anti-APC Antibody (CAB2818)Immunofluorescence analysis of HT-29 cells using APC antibody (CAB2818) at dilution of 1:100. Blue: DAPI for nuclear staining.
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