Name: Anti-AP3M1 Antibody (CAB10410)
Brand: Immunology Antibodies 1
SKU: CAB10410
Availability: OutOfStock

Antibody Name:	Anti-AP3M1 Antibody
Antibody SKU:	CAB10410
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 219-418 of human AP3M1 (NP_036227.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, Mouse liver, Mouse kidney, Rat spinal cord, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 219-418 of human AP3M1 (NP_036227.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLSF MNPR LLDD VSFH PCIR FKRW ESER VLSF IPPD GNFR LISY RVSS QNLV AIPV YVKH SISF KENS SCGR FDIT IGPK QNMG KTIE GITV TVHM PKVV LNMN LTPT QGSY TFDP VTKV LTWD VGKI TPQK LPSL KGLV NLQS GAPK PEEN PSLN IQFK IQQL AISG LKVN RLDM YGEK YKPF KGVK YVTK AGKF QVRT
Gene ID:	26985
Uniprot:	Q9Y2T2
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle membrane, Golgi apparatus, Peripheral membrane protein
Calculated MW:	46kDa
Observed MW:	47kDa

Synonyms:

AP3M1

Background:

The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane, and it may directly function in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	AP3M1: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Belongs to the adaptor complexes medium subunit family.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 10q22.2 Cellular Component: lysosomal membrane; lysosome Molecular Function:protein binding; Rab GTPase binding Biological Process: anterograde axon cargo transport; anterograde synaptic vesicle transport; protein targeting to lysosome
NCBI Summary:	The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane, and it may directly function in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:	Q9Y2T2
NCBI GenInfo Identifier:	13123952
NCBI Gene ID:	26985
NCBI Accession:	Q9Y2T2.1
UniProt Secondary Accession:	Q9Y2T2,Q5JQ12, Q9H5L2,
UniProt Related Accession:	Q9Y2T2
Molecular Weight:	46,939 Da
NCBI Full Name:	AP-3 complex subunit mu-1
NCBI Synonym Full Names:	adaptor related protein complex 3 mu 1 subunit
NCBI Official Symbol:	AP3M1
NCBI Protein Information:	AP-3 complex subunit mu-1
UniProt Protein Name:	AP-3 complex subunit mu-1
UniProt Synonym Protein Names:	AP-3 adaptor complex mu3A subunit; Adaptor-related protein complex 3 subunit mu-1; Mu-adaptin 3A; Mu3A-adaptin
Protein Family:	AP-3 complex
UniProt Gene Name:	AP3M1
UniProt Entry Name:	AP3M1_HUMAN

Western blot analysis of extracts of various cell lines, using AP3M1 antibody (CAB10410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

AP3M1 Antibody (PACO07770)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)