Cell Biology Antibodies 4
Anti-AP3B2 Antibody (CAB13808)
(No reviews yet)
Write a Review
- SKU:
- CAB13808
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AP3B2 Antibody |
| Antibody SKU: | CAB13808 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 833-1082 of human AP3B2 (NP_004635.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 833-1082 of human AP3B2 (NP_004635.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AADL EGLT LTDS TLVP SLLS PVSG VGRQ ELLH RVAG EGLA VDYT FSRQ PFSG DPHM VSVH IHFS NSSD TPIK GLHV GTPK LPAG ISIQ EFPE IESL APGE SATA VMGI NFCD STQA ANFQ LCTQ TRQF YVSI QPPV GELM APVF MSEN EFKK EQGK LMGM NEIT EKLM LPDT CRSD HIVV QKVT ATAN LGRV PCGT SDEY RFAG RTLT GGSL VLLT LDAR PAGA AQLT VNSE KMVI GTML VKDV IQAL TQ |
| Gene ID: | 8120 |
| Uniprot: | Q13367 |
| Cellular Location: | Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane |
| Calculated MW: | 15kDa/115kDa/119kDa/121kDa |
| Observed MW: | 130kDa |
| Synonyms: | AP3B2, EIEE48, NAPTB |
| Background: | Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. |
| UniProt Protein Function: | AP3B2: Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 15q Cellular Component: COPI-coated vesicle; cytoplasm; Golgi apparatus; nucleoplasm Molecular Function:transporter activity Biological Process: anterograde axon cargo transport; anterograde synaptic vesicle transport; post-Golgi vesicle-mediated transport |
| NCBI Summary: | Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed, whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex, which includes AP3B2, is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al., 2006 [PubMed 16788073]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q13367 |
| NCBI GenInfo Identifier: | 18202497 |
| NCBI Gene ID: | 8120 |
| NCBI Accession: | Q13367.2 |
| UniProt Secondary Accession: | Q13367,O14808, Q52LY8, A4Z4T7, B7ZKR7, B7ZKS0, |
| UniProt Related Accession: | Q13367 |
| Molecular Weight: | 121,189 Da |
| NCBI Full Name: | AP-3 complex subunit beta-2 |
| NCBI Synonym Full Names: | adaptor related protein complex 3 beta 2 subunit |
| NCBI Official Symbol: | AP3B2 |
| NCBI Official Synonym Symbols: | NAPTB |
| NCBI Protein Information: | AP-3 complex subunit beta-2 |
| UniProt Protein Name: | AP-3 complex subunit beta-2 |
| UniProt Synonym Protein Names: | Adaptor protein complex AP-3 subunit beta-2; Adaptor-related protein complex 3 subunit beta-2; Beta-3B-adaptin; Clathrin assembly protein complex 3 beta-2 large chain; Neuron-specific vesicle coat protein beta-NAP |
| Protein Family: | AP-3 complex |
| UniProt Gene Name: | AP3B2 |
| UniProt Entry Name: | AP3B2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AP3B2 antibody (CAB13808) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
View AllClose
Related Products
