Antibody Name:	Anti-AP2M1 Antibody
Antibody SKU:	CAB13962
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human AP2M1 (NP_001020376.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 134-433 of human AP2M1 (NP_001020376.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TQQG IKSQ TKEE QSQI TSQV TGQI GWRR EGIK YRRN ELFL DVLE SVNL LMSP QGQV LSAH VSGR VVMK SYLS GMPE CKFG MNDK IVIE KQGK GTAD ETSK SGKQ SIAI DDCT FHQC VRLS KFDS ERSI SFIP PDGE FELM RYRT TKDI ILPF RVIP LVRE VGRT KLEV KVVI KSNF KPSL LAQK IEVR IPTP LNTS GVQV ICMK GKAK YKAS ENAI VWKI KRMA GMKE SQIS AEIE LLPT NDKK KWAR PPIS MNFE VPFA PSGL KVRY LKVF EPKL NYSD HDVI KWVR YIGR SGIY ETRC
Gene ID:	1173
Uniprot:	Q96CW1
Cellular Location:	Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit
Calculated MW:	49kDa
Observed MW:	50KDa

Synonyms:

AP2M1, AP50, CLAPM1, mu2

Background:

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).
UniProt Protein Details:	Protein type:Vesicle; Adaptor/scaffold Chromosomal Location of Human Ortholog: 3q28 Cellular Component: mitochondrion; lysosomal membrane; plasma membrane; AP-2 adaptor complex; cytosol Molecular Function:protein binding; low-density lipoprotein receptor binding; transporter activity; signal sequence binding; lipid binding Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus
NCBI Summary:	This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96CW1
NCBI GenInfo Identifier:	51316978
NCBI Gene ID:	1173
NCBI Accession:	Q96CW1.2
UniProt Secondary Accession:	Q96CW1,P20172, P53679, A6NE12, D3DNT1,
UniProt Related Accession:	Q96CW1
Molecular Weight:	435
NCBI Full Name:	AP-2 complex subunit mu
NCBI Synonym Full Names:	adaptor-related protein complex 2, mu 1 subunit
NCBI Official Symbol:	AP2M1
NCBI Official Synonym Symbols:	mu2; AP50; CLAPM1
NCBI Protein Information:	AP-2 complex subunit mu; adaptin-mu2; AP-2 mu chain; AP-2 mu 2 chain; HA2 50 kDA subunit; clathrin coat adaptor protein AP50; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50; adaptor protein complex AP-2 subunit mu; clathrin ada
UniProt Protein Name:	AP-2 complex subunit mu
UniProt Synonym Protein Names:	AP-2 mu chain; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; Adaptor-related protein complex 2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; HA2 50 kDa subunit; Plasma membrane adaptor AP-2 50 kDa protein
UniProt Gene Name:	AP2M1
UniProt Entry Name:	AP2M1_HUMAN

	Western blot analysis of extracts of various cell lines, using AP2M1 antibody (CAB13962) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of HeLa cells, using AP2M1 antibody (CAB13962) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of HeLa cells using AP2M1 antibody (CAB13962). Blue: DAPI for nuclear staining.