Immunology Antibodies 1

Anti-AP1M1 Antibody (CAB10129)

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SKU:
CAB10129
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-AP1M1 Antibody
Antibody SKU:CAB10129
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human AP1M1 (NP_115882.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, HepG2, HL-60, HT-1080, Mouse brain, Mouse kidney, Mouse liver, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human AP1M1 (NP_115882.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAS AVYV LDLK GKVL ICRN YRGD VDMS EVEH FMPI LMEK EEEG MLSP ILAH GGVR FMWI KHNN LYLV ATSK KNAC VSLV FSFL YKVV QVFS EYFK ELEE ESIR DNFV IIYE LLDE LMDF GYPQ TTDS KILQ EYIT QEGH KLET GAPR PPAT VTNA VSWR
Gene ID:8907
Uniprot:Q9BXS5
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane
Calculated MW:48kDa/49kDa
Observed MW:48kDa
Synonyms:AP1M1, AP47, CLAPM2, CLTNM, MU-1A
Background:The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
UniProt Protein Function:AP1M1: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes medium subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic vesicle membrane; cytosol; Golgi membrane; lysosomal membrane; membrane; trans-Golgi network membrane

Molecular Function:protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; melanosome organization and biogenesis; regulation of defense response to virus by virus

NCBI Summary:The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BXS5
NCBI GenInfo Identifier:18202738
NCBI Gene ID:8907
NCBI Accession:Q9BXS5.3
UniProt Secondary Accession:Q9BXS5,Q4TTY5,
UniProt Related Accession:Q9BXS5
Molecular Weight:49,840 Da
NCBI Full Name:AP-1 complex subunit mu-1
NCBI Synonym Full Names:adaptor related protein complex 1 mu 1 subunit
NCBI Official Symbol:AP1M1  
NCBI Official Synonym Symbols:AP47; CLTNM; MU-1A; CLAPM2  
NCBI Protein Information:AP-1 complex subunit mu-1
UniProt Protein Name:AP-1 complex subunit mu-1
UniProt Synonym Protein Names:AP-mu chain family member mu1A; Adaptor protein complex AP-1 subunit mu-1; Adaptor-related protein complex 1 subunit mu-1; Clathrin assembly protein complex 1 mu-1 medium chain 1; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; Mu-adaptin 1; Mu1A-adaptin
Protein Family:AP-1 complex
UniProt Gene Name:AP1M1  
UniProt Entry Name:AP1M1_HUMAN
Anti-AP1M1 Antibody (CAB10129)Western blot analysis of extracts of various cell lines, using AP1M1 antibody (CAB10129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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