Antibody Name:	Anti-ANXA5 Antibody [KO Validated]
Antibody SKU:	CAB1728
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2, HeLa, DU145, PC-3, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQV LRGT VTDF PGFD ERAD AETL RKAM KGLG TDEE SILT LLTS RSNA QRQE ISAA FKTL FGRD LLDD LKSE LTGK FEKL IVAL MKPS RLYD AYEL KHAL KGAG TNEK VLTE IIAS RTPE ELRA IKQV YEEE YGSS LEDD VVGD TSGY YQRM LVVL LQAN RDPD AGID EAQV EQDA QALF QAGE LKWG TDEE KFIT IFGT RSVS HLRK VFDK YMTI SGFQ IEET IDRE TSGN LEQL LLAV VKSI RSIP AYLA ETLY YAMK GAGT DDHT LIRV MVSR SEID LFNI RKEF RKNF ATSL YSMI KGDT SGDY KKAL LLLC GEDD
Gene ID:	308
Uniprot:	P08758
Cellular Location:
Calculated MW:	35kDa
Observed MW:	35kDa

Synonyms:

ANXA5, ANX5, ENX2, HEL-S-7, PP4, RPRGL3

Background:

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

UniProt Protein Function:	This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
NCBI Summary:	The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
UniProt Code:	P08758
NCBI GenInfo Identifier:	113960
NCBI Gene ID:	308
NCBI Accession:	P08758.2
UniProt Secondary Accession:	P08758,Q6FHB3, Q6FI16, Q8WV69, Q9UDH9, D3DNW7,
UniProt Related Accession:	P08758
Molecular Weight:	35,937 Da
NCBI Full Name:	Annexin A5
NCBI Synonym Full Names:	annexin A5
NCBI Official Symbol:	ANXA5
NCBI Official Synonym Symbols:	PP4; ANX5; ENX2; RPRGL3; HEL-S-7
NCBI Protein Information:	annexin A5
UniProt Protein Name:	Annexin A5
UniProt Synonym Protein Names:	Anchorin CII; Annexin V; Annexin-5; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; PP4; Placental anticoagulant protein I; PAP-I; Thromboplastin inhibitor; Vascular anticoagulant-alpha; VAC-alpha
Protein Family:	Annexin
UniProt Gene Name:	ANXA5
UniProt Entry Name:	ANXA5_HUMAN

	Western blot analysis of extracts of various cell lines, using ANXA5 antibody (CAB1728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and ANXA5 knockout (KO) 293T cells, using ANXA5 antibody (CAB1728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA5 Rabbit pAb (CAB1728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ANXA5 Antibody (CAB13945)	Human Annexin A5 / ANXA5 ELISA Kit
Anti-Annexin V Antibody (CAB9262)	Human ANXA5 (Annexin A5) ELISA Kit (HUES01635)
ANXA5 Antibody (PACO07755)	Human Annexin A5 (ANXA5) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)