Antibody Name:	Anti-ANXA11 Antibody
Antibody SKU:	CAB7423
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 (NP_001148.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, HT-29, Jurkat, SKOV3, A-549, Mouse brain, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 (NP_001148.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FDIY EIKE AIKG VGTD EACL IEIL ASRS NEHI RELN RAYK AEFK KTLE EAIR SDTS GHFQ RLLI SLSQ GNRD ESTN VDMS LAQR DAQE LYAA GENR LGTD ESKF NAVL CSRS RAHL VAVF NEYQ RMTG RDIE KSIC REMS GDLE EGML AVVK CLKN TPAF FAER LNKA MRGA GTKD RTLI RIMV SRSE TDLL DIRS EYKR MYGK SLYH DISG DTSG DYRK ILLK ICGG ND
Gene ID:	311
Uniprot:	P50995
Cellular Location:	Cytoplasm, Melanosome, Nucleus, Nucleus envelope, cytoskeleton, nucleoplasm, spindle
Calculated MW:	51kDa/54kDa
Observed MW:	56kDa

Synonyms:

ANXA11, ANX11, CAP50

Background:

This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.

UniProt Protein Function:	ANXA11: a calcium/phospholipid-binding protein found throughout the nucleoplasm at interphase and at the mitotic phase is concentrated at the loop-like structure around the mitotic apparatus. May interact with calcyclin (S100A6) in vivo. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
UniProt Protein Details:	Protein type:Nuclear envelope; Lipid-binding; Calcium-binding; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10q23 Cellular Component: nucleoplasm; azurophil granule; specific granule; membrane; cytoplasm; melanosome; spindle; nuclear envelope; phagocytic vesicle; midbody Molecular Function:protein binding; phosphatidylethanolamine binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding Biological Process: response to calcium ion; phagocytosis
NCBI Summary:	This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, May 2013]
UniProt Code:	P50995
NCBI GenInfo Identifier:	1703322
NCBI Gene ID:	311
NCBI Accession:	P50995.1
UniProt Secondary Accession:	P50995,B4DVE7,
UniProt Related Accession:	P50995
Molecular Weight:	51,242 Da
NCBI Full Name:	Annexin A11
NCBI Synonym Full Names:	annexin A11
NCBI Official Symbol:	ANXA11
NCBI Official Synonym Symbols:	ANX11; CAP50
NCBI Protein Information:	annexin A11; CAP-50; annexin XI; annexin-11; 56 kDa autoantigen; autoantigen, 56-kD; calcyclin-associated annexin 50
UniProt Protein Name:	Annexin A11
UniProt Synonym Protein Names:	56 kDa autoantigen; Annexin XI; Annexin-11; Calcyclin-associated annexin 50; CAP-50
Protein Family:	Annexin
UniProt Gene Name:	ANXA11
UniProt Entry Name:	ANX11_HUMAN

	Western blot analysis of extracts of various cell lines, using ANXA11 antibody (CAB7423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using ANXA11 antibody (CAB7423). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody (CAB7423). Western blot was performed from the immunoprecipitate using ANXA11 antibody (CAB7423) at a dilition of 1:1000.