Name: Anti-ANXA1 Antibody (CAB1118)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1118
Availability: OutOfStock

Antibody Name:	Anti-ANXA1 Antibody [KO Validated]
Antibody SKU:	CAB1118
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMV SEFL KQAW FIEN EEQE YVQT VKSS KGGP GSAV SPYP TFNP SSDV AALH KAIM VKGV DEAT IIDI LTKR NNAQ RQQI KAAY LQET GKPL DETL KKAL TGHL EEVV LALL KTPA QFDA DELR AAMK GLGT DEDT LIEI LASR TNKE IRDI NRVY REEL KRDL AKDI TSDT SGDF RNAL LSLA KGDR SEDF GVNE DLAD SDAR ALYE AGER RKGT DVNV FNTI LTTR SYPQ LRRV FQKY TKYS KHDM NKVL DLEL KGDI EKCL TAIV KCAT SKPA FFAE KLHQ AMKG VGTR HKAL IRIM VSRS EIDM NDIK AFYQ KMYG ISLC QAIL DETK GDYE KILV ALCG GN
Gene ID:	301
Uniprot:	P04083
Cellular Location:	Apical cell membrane, Basolateral cell membrane, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Early endosome, Endosome membrane, Extracellular side, Lateral cell membrane, Membrane, Nucleus, Peripheral membrane protein, Secreted, cilium, exosome, extracellular space, phagocytic cup, secretory vesicle lumen
Calculated MW:	38kDa
Observed MW:	38kDa

Synonyms:	ANXA1, ANX1, LPC1
Background:	This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.

UniProt Protein Function:	ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
UniProt Protein Details:	Protein type:Lipid-binding; Calcium-binding Chromosomal Location of Human Ortholog: 9q21.13 Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; cornified envelope; cytoplasm; cytoplasmic vesicle membrane; early endosome membrane; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; extrinsic to membrane; focal adhesion; lateral plasma membrane; mast cell granule; mitochondrial membrane; nucleoplasm; nucleus; phagocytic cup; plasma membrane; protein complex; sarcolemma; vesicle Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; double-stranded DNA-dependent ATPase activity; helicase activity; phospholipase A2 inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; protein homodimerization activity; receptor binding; single-stranded DNA binding; single-stranded RNA binding; structural molecule activity Biological Process: actin cytoskeleton reorganization; adaptive immune response; alpha-beta T cell differentiation; arachidonic acid secretion; cell surface receptor linked signal transduction; DNA duplex unwinding; DNA strand renaturation; endocrine pancreas development; G-protein signaling, coupled to cyclic nucleotide second messenger; gliogenesis; inflammatory response; innate immune response; insulin secretion; keratinocyte differentiation; monocyte chemotaxis; muscle contraction; myoblast migration involved in skeletal muscle regeneration; negative regulation of apoptosis; negative regulation of exocytosis; negative regulation of T-helper 2 cell differentiation; neutrophil homeostasis; peptide cross-linking; phagocytosis; positive regulation of interleukin-2 production; positive regulation of neutrophil apoptosis; positive regulation of prostaglandin biosynthetic process; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of vesicle fusion; prostate gland development; regulation of cell shape; regulation of hormone secretion; regulation of inflammatory response; regulation of interleukin-1 production; regulation of leukocyte migration; response to drug; response to estradiol stimulus; response to peptide hormone stimulus; response to X-ray; signal transduction
NCBI Summary:	This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
UniProt Code:	P04083
NCBI GenInfo Identifier:	113944
NCBI Gene ID:	301
NCBI Accession:	P04083.2
UniProt Secondary Accession:	P04083,P10107,
UniProt Related Accession:	P04083
Molecular Weight:	38,714 Da
NCBI Full Name:	Annexin A1
NCBI Synonym Full Names:	annexin A1
NCBI Official Symbol:	ANXA1
NCBI Official Synonym Symbols:	ANX1; LPC1
NCBI Protein Information:	annexin A1
UniProt Protein Name:	Annexin A1
UniProt Synonym Protein Names:	Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I
Protein Family:	Annexin
UniProt Gene Name:	ANXA1
UniProt Entry Name:	ANXA1_HUMAN

	Western blot analysis of extracts of various cell lines, using ANXA1 antibody (CAB1118). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (CAB1118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (CAB1118) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (CAB1118) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ANXA1 antibody (CAB1118). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Phospho-ANXA1 (Y21) Antibody (PACO00335)	Human Annexin A1 ELISA Kit
ANXA1 Antibody (PACO02808)	Human Annexin A1 (ANXA1) ELISA Kit
ANXA1 Antibody (PACO06249)
ANXA1 Antibody (PACO07749)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)