Name: Anti-ANT1/ANT2/ANT3/ANT4 Antibody (CAB15988)
Brand: Cell Biology Antibodies 6
SKU: CAB15988
Availability: OutOfStock

Antibody Name:	Anti-ANT1/ANT2/ANT3/ANT4 Antibody
Antibody SKU:	CAB15988
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human ANT1 (NP_001142.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse skeletal muscle, Rat heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human ANT1 (NP_001142.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GMLP DPKN VHIF VSWM IAQS VTAV AGLV SYPF DTVR RRMM MQSG RKGA DIMY TGTV DCWR KIAK DEGA KAFF KGAW SNVL RGMG GAFV LVLY DEIK KYV
Gene ID:	291/292/293/83447
Uniprot:	P12235/P05141/P12236/Q9H0C2
Cellular Location:
Calculated MW:	33kDa/32kDa/35kDa
Observed MW:	30kDa

Synonyms:	ANT1/ANT2/ANT3/ANT4
Background:

UniProt Protein Function:	SLC25A4: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. Defects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2). Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged- red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. Belongs to the mitochondrial carrier family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family; Mitochondrial Chromosomal Location of Human Ortholog: 4q35 Cellular Component: mitochondrion; integral to plasma membrane; mitochondrial inner membrane; nucleus Molecular Function:protein binding; adenine transmembrane transporter activity Biological Process: adenine transport; mitochondrial genome maintenance; apoptotic mitochondrial changes; generation of precursor metabolites and energy; viral reproduction; transport; energy reserve metabolic process; regulation of insulin secretion; transmembrane transport Disease: Mitochondrial Dna Depletion Syndrome 12 (cardiomyopathic Type); Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Dominant, 2
NCBI Summary:	This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy. [provided by RefSeq, Jun 2013]
UniProt Code:	P12235
NCBI GenInfo Identifier:	113455
NCBI Gene ID:	291
NCBI Accession:	P12235.4
UniProt Secondary Accession:	P12235,D3DP59,
UniProt Related Accession:	P12235
Molecular Weight:	298
NCBI Full Name:	ADP/ATP translocase 1
NCBI Synonym Full Names:	solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
NCBI Official Symbol:	SLC25A4
NCBI Official Synonym Symbols:	1; T1; ANT; AAC1; ANT1; PEO2; PEO3; ANT 1; MTDPS12
NCBI Protein Information:	ADP/ATP translocase 1; ADP,ATP carrier protein 1; solute carrier family 25 member 4; heart/skeletal muscle ATP/ADP translocator; ADP,ATP carrier protein, heart/skeletal muscle; adenine nucleotide translocator 1 (skeletal muscle)
UniProt Protein Name:	ADP/ATP translocase 1
UniProt Synonym Protein Names:	ADP,ATP carrier protein 1; ADP,ATP carrier protein, heart/skeletal muscle isoform T1; Adenine nucleotide translocator 1; ANT 1; Solute carrier family 25 member 4
Protein Family:	ADP/ATP translocase
UniProt Gene Name:	SLC25A4
UniProt Entry Name:	ADT1_HUMAN

	Western blot analysis of extracts of various cell lines, using ANT1/ANT2/ANT3/ANT4 antibody (CAB15988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 150s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat kidney using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at dilution of 1:50 (40x lens).