Cardiovascular Antibodies
Anti-ANGPT2 Antibody (CAB0698)
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- SKU:
- CAB0698
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-ANGPT2 Antibody |
| Antibody SKU: | CAB0698 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 197-496 of human ANGPT2 (NP_001138.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HT-29, HepG2, K-562, Raji, Mouse brain, Mouse spleen, Rat kidney, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 197-496 of human ANGPT2 (NP_001138.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AMED KHII QLQS IKEE KDQL QVLV SKQN SIIE ELEK KIVT ATVN NSVL QKQQ HDLM ETVN NLLT MMST SNSA KDPT VAKE EQIS FRDC AEVF KSGH TTNG IYTL TFPN STEE IKAY CDME AGGG GWTI IQRR EDGS VDFQ RTWK EYKV GFGN PSGE YWLG NEFV SQLT NQQR YVLK IHLK DWEG NEAY SLYE HFYL SSEE LNYR IHLK GLTG TAGK ISSI SQPG NDFS TKDG DNDK CICK CSQM LTGG WWFD ACGP SNLN GMYY PQRQ NTNK FNGI KWYY WKGS GYSL KATT MMIR PADF |
| Gene ID: | 285 |
| Uniprot: | O15123 |
| Cellular Location: | Secreted |
| Calculated MW: | 50kDa/56kDa |
| Observed MW: | 75kDa |
| Synonyms: | ANGPT2, AGPT2, ANG2 |
| Background: | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. |
| UniProt Protein Function: | ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8p23.1 Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus Molecular Function:protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; signal transduction; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; negative regulation of angiogenesis; positive regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; angiogenesis; response to activity; blood coagulation; leukocyte migration |
| NCBI Summary: | The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15123 |
| NCBI GenInfo Identifier: | 12229555 |
| NCBI Gene ID: | 285 |
| NCBI Accession: | O15123.1 |
| UniProt Secondary Accession: | O15123,Q9NRR7, Q9P2Y7, A0AV38, A8K205, B7ZLM7, |
| UniProt Related Accession: | O15123 |
| Molecular Weight: | 56,848 Da |
| NCBI Full Name: | Angiopoietin-2 |
| NCBI Synonym Full Names: | angiopoietin 2 |
| NCBI Official Symbol: | ANGPT2 |
| NCBI Official Synonym Symbols: | ANG2; AGPT2 |
| NCBI Protein Information: | angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a |
| UniProt Protein Name: | Angiopoietin-2 |
| UniProt Gene Name: | ANGPT2 |
| UniProt Entry Name: | ANGP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ANGPT2 antibody (CAB0698) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of HeLa cells using ANGPT2 antibody (CAB0698) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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