Cardiovascular Antibodies

Anti-ANG Antibody (CAB13465)

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SKU:
CAB13465
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-ANG Antibody
Antibody SKU:CAB13465
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human ANG (NP_001091046.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, LO2, HeLa, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-147 of human ANG (NP_001091046.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QDNS RYTH FLTQ HYDA KPQG RDDR YCES IMRR RGLT SPCK DINT FIHG NKRS IKAI CENK NGNP HREN LRIS KSSF QVTT CKLH GGSP WPPC QYRA TAGF RNVV VACE NGLP VHLD QSIF RRP
Gene ID:283
Uniprot:P03950
Cellular Location:Nucleus, Secreted, basement membrane, extracellular matrix, extracellular space, nucleolus
Calculated MW:16kDa
Observed MW:13kDa
Synonyms:ANG, ALS9, HEL168, RAA1, RNASE4, RNASE5
Background:The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.
UniProt Protein Function:ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.
UniProt Protein Details:

Protein type:RNA-binding; Secreted, signal peptide; Ribonuclease; EC 3.1.27.-; Secreted; Motility/polarity/chemotaxis; Nucleolus; Extracellular matrix; Cell cycle regulation

Chromosomal Location of Human Ortholog: 14q11.1-q11.2

Cellular Component: angiogenin-PRI complex; basal lamina; cell soma; cytoplasmic membrane-bound vesicle; extracellular region; extracellular space; growth cone; nucleolus; nucleus

Molecular Function:actin binding; copper ion binding; DNA binding; endonuclease activity; heparin binding; peptide binding; protein binding; protein homodimerization activity; receptor binding; ribonuclease activity; rRNA binding

Biological Process: actin filament polymerization; activation of phospholipase A2; activation of protein kinase B; angiogenesis; antibacterial humoral response; antifungal humoral response; cell communication; cell migration; defense response to Gram-positive bacterium; diacylglycerol biosynthetic process; homeostatic process; innate immune response; intercellular junction assembly and maintenance; negative regulation of smooth muscle cell proliferation; negative regulation of translation; oocyte maturation; ovarian follicle development; phospholipase C activation; placenta development; positive regulation of endothelial cell proliferation; positive regulation of phosphorylation; positive regulation of protein secretion; response to hormone stimulus; response to hypoxia; response to yeast; rRNA transcription

Disease: Amyotrophic Lateral Sclerosis 9

NCBI Summary:The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]
UniProt Code:P03950
NCBI GenInfo Identifier:113873
NCBI Gene ID:283
NCBI Accession:P03950.1
UniProt Secondary Accession:P03950,Q05CV1, Q53X86, Q6P5T2, Q8WXE7,
UniProt Related Accession:P03950
Molecular Weight:16,550 Da
NCBI Full Name:Angiogenin
NCBI Synonym Full Names:angiogenin, ribonuclease, RNase A family, 5
NCBI Official Symbol:ANG  
NCBI Official Synonym Symbols:ALS9; RAA1; HEL168; RNASE4; RNASE5  
NCBI Protein Information:angiogenin
UniProt Protein Name:Angiogenin
UniProt Synonym Protein Names:Ribonuclease 5; RNase 5
Protein Family:Angiogenin
UniProt Gene Name:ANG  
UniProt Entry Name:ANGI_HUMAN
Anti-ANG Antibody (CAB13465)Western blot analysis of extracts of various cell lines, using ANG antibody (CAB13465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-ANG Antibody (CAB13465)Immunofluorescence analysis of NIH/3T3 cells using ANG Rabbit pAb (CAB13465) at dilution of 1:100. Blue: DAPI for nuclear staining.
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