Antibody Name:	Anti-ANAPC5 Antibody
Antibody SKU:	CAB7109
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, MCF-7, HL-60, 22Rv1, A-549, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANAPC5 (NP_057321.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASV HESL YFNP MMTN GVVH ANVF GIKD WVTP YKIA VLVL LNEM SRTG EGAV SLME RRRL NQLL LPLL QGPD ITLS KLYK LIEE SCPQ LANS VQIR IKLM AEGE LKDM EQFF DDLS DSFS GTEP EVHK TSVV GLFL RHMI LAYS KLSF SQVF KLYT ALQQ YFQN GEKK TVED ADME LTSR DEGE RKME KEEL DVSV REEE VSCS GPLS QKQA EFFL SQQA SLLK NDET KALT PASL QKEL NNLL KFNP DF
Gene ID:	51433
Uniprot:	Q9UJX4
Cellular Location:
Calculated MW:	25kDa/72kDa/85kDa
Observed MW:	85kDa

Synonyms:

ANAPC5, APC5

Background:

This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.

UniProt Protein Function:	APC5: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol Molecular Function:protein phosphatase binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
NCBI Summary:	This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]
UniProt Code:	Q9UJX4
NCBI GenInfo Identifier:	37537861
NCBI Gene ID:	51433
NCBI Accession:	Q9UJX4.2
UniProt Secondary Accession:	Q9UJX4,Q8N4H7, Q9BQD4, E9PFB2,
UniProt Related Accession:	Q9UJX4
Molecular Weight:	72,526 Da
NCBI Full Name:	Anaphase-promoting complex subunit 5
NCBI Synonym Full Names:	anaphase promoting complex subunit 5
NCBI Official Symbol:	ANAPC5
NCBI Official Synonym Symbols:	APC5
NCBI Protein Information:	anaphase-promoting complex subunit 5; cyclosome subunit 5
UniProt Protein Name:	Anaphase-promoting complex subunit 5
UniProt Synonym Protein Names:	Cyclosome subunit 5
UniProt Gene Name:	ANAPC5
UniProt Entry Name:	APC5_HUMAN

	Western blot analysis of extracts of various cell lines, using ANAPC5 antibody (CAB7109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using ANAPC5 antibody (CAB7109) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug ANAPC5 antibody (CAB7109). Western blot was performed from the immunoprecipitate using ANAPC5 antibody (CAB7109) at a dilition of 1:1000.