ChIP Antibodies
Anti-AMY2A Antibody (CAB8045)
- SKU:
- CAB8045
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | AMY2A Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8045 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse intestine, Rat intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-511 of human AMY2A (NP_000690.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KPFI YQEV IDLG GEPI KSSD YFGN GRVT EFKY GAKL GTVI RKWN GEKM SYLK NWGE GWGF VPSD RALV FVDN HDNQ RGHG AGGA SILT FWDA RLYK MAVG FMLA HPYG FTRV MSSY RWPR QFQN GNDV NDWV GPPN NNGV IKEV TINP DTTC GNDW VCEH RWRQ IRNM VIFR NVVD GQPF TNWY DNGS NQVA FGRG NRGF IVFN NDDW SFSL TLQT GLPA GTYC DVIS GDKI NGNC TGIK IYVS DDGK AHFS ISNS AEDP FIAI HAES KL |
| Gene ID: | 279 |
| Uniprot: | P04746 |
| Cellular Location: | Secreted, extracellular space |
| Calculated MW: | 30kDa/57kDa |
| Observed MW: | 58kDa |
| Synonyms: | AMY2A, AMY2, PA |
| Background: | This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. |
| UniProt Protein Function: | AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Secreted; Hydrolase; EC 3.2.1.1; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose Chromosomal Location of Human Ortholog: 1p21 Cellular Component: extracellular space; extracellular region Molecular Function:alpha-amylase activity; calcium ion binding; chloride ion binding Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis |
| NCBI Summary: | This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015] |
| UniProt Code: | P04746 |
| NCBI GenInfo Identifier: | 113803 |
| NCBI Gene ID: | 279 |
| NCBI Accession: | P04746.2 |
| UniProt Secondary Accession: | P04746,Q9UBH3, B9EJG1, |
| UniProt Related Accession: | P04746 |
| Molecular Weight: | 511 |
| NCBI Full Name: | Pancreatic alpha-amylase |
| NCBI Synonym Full Names: | amylase, alpha 2A (pancreatic) |
| NCBI Official Symbol: | AMY2A |
| NCBI Official Synonym Symbols: | PA; AMY2 |
| NCBI Protein Information: | pancreatic alpha-amylase; glycogenase; pancreatic amylase alpha 2A; 1,4-alpha-D-glucan glucanohydrolase |
| UniProt Protein Name: | Pancreatic alpha-amylase |
| UniProt Synonym Protein Names: | 1,4-alpha-D-glucan glucanohydrolase |
| UniProt Gene Name: | AMY2A |
| UniProt Entry Name: | AMYP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMY2A antibody (CAB8045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using AMY2A antibody (CAB8045) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using AMY2A antibody (CAB8045) at dilution of 1:100 (40x lens). |
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