Description
| Antibody Name: | Anti-AMY1A Antibody |
| Antibody SKU: | CAB13276 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human AMY1A (NP_004029.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-200 of human AMY1A (NP_004029.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QYSS NTQQ GRTS IVHL FEWR WVDI ALEC ERYL APKG FGGV QVSP PNEN VAIH NPFR PWWE RYQP VSYK LCTR SGNE DEFR NMVT RCNN VGVR IYVD AVIN HMCG NAVS AGTS STCG SYFN PGSR DFPA VPYS GWDF NDGK CKTG SGDI ENYN DATQ VRDC RLSG LLDL ALGK DYVR SKIA EYMN H |
| Gene ID: | 276 |
| Uniprot: | P04745 |
| Cellular Location: | Secreted |
| Calculated MW: | 57kDa |
| Observed MW: | 54kDa |
| Synonyms: | AMY1A, AMY1 |
| Background: | Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. |
| UniProt Protein Function: | AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family. |
| UniProt Protein Details: | Protein type:Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted; EC 3.2.1.1; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p21 Cellular Component: extracellular space Molecular Function:alpha-amylase activity; protein binding; metal ion binding Biological Process: carbohydrate metabolic process; digestion |
| NCBI Summary: | Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04745 |
| NCBI GenInfo Identifier: | 1351933 |
| NCBI Gene ID: | 277 |
| NCBI Accession: | P04745.2 |
| UniProt Secondary Accession: | P04745,Q13763, Q5T083, A6NJS5, A8K8H6, |
| UniProt Related Accession: | P04745 |
| Molecular Weight: | |
| NCBI Full Name: | Alpha-amylase 1 |
| NCBI Synonym Full Names: | amylase, alpha 1B (salivary) |
| NCBI Official Symbol: | AMY1B |
| NCBI Official Synonym Symbols: | AMY1 |
| NCBI Protein Information: | alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1 |
| UniProt Protein Name: | Alpha-amylase 1 |
| UniProt Synonym Protein Names: | 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase |
| UniProt Gene Name: | AMY1A |
| UniProt Entry Name: | AMY1_HUMAN |
 | Western blot analysis of extracts of mouse liver, using AMY1A antibody (CAB13276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using AMY1A antibody (CAB13276) at dilution of 1:100 (40x lens). |
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