ChIP Antibodies
Anti-AMT Antibody (CAB9926)
- SKU:
- CAB9926
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AMT Antibody |
| Antibody SKU: | CAB9926 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, SW620, 22Rv1, Mouse pancreas, Mouse brain, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 107-386 of human AMT (NP_001158184.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AELR PNQG TLSL FTNE AGGI LDDL IVTN TSEG HLYV VSNA GCWE KDLA LMQD KVRE LQNQ GRDV GLEV LDNA LLAL QGPT AAQV LQAG VADD LRKL PFMT SAVM EVFG VSGC RVTR CGYT GEDG VEIS VPVA GAVH LATA ILKN PEVK LAGL AARD SLRL EAGL CLYG NDID EHTT PVEG SLSW TLGK RRRA AMDF PGAK VIVP QLKG RVQR RRVG LMCE GAPM RAHS PILN MEGT KIGT VTSG CPSP SLKK NVAM GYVP CEYS RPGT MLLV ELPS GPCF |
| Gene ID: | 275 |
| Uniprot: | P48728 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 37kDa/39kDa/41kDa/43kDa |
| Observed MW: | 44kDa |
| Synonyms: | AMT, GCE, GCST, GCVT, NKH |
| Background: | This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | AMT: The glycine cleavage system catalyzes the degradation of glycine. Defects in AMT are a cause of non-ketotic hyperglycinemia (NKH); also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. Belongs to the GcvT family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - nitrogen; Methyltransferase; Mitochondrial; EC 2.1.2.10; Cofactor and Vitamin Metabolism - one carbon pool by folate Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:aminomethyltransferase activity Biological Process: glycine catabolic process; glycine decarboxylation via glycine cleavage system; glyoxylate metabolic process Disease: Glycine Encephalopathy |
| NCBI Summary: | This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P48728 |
| NCBI GenInfo Identifier: | 1346122 |
| NCBI Gene ID: | 275 |
| NCBI Accession: | P48728.1 |
| UniProt Secondary Accession: | P48728,Q96IG6, A8K3I5, B4DE61, B4DJQ0, E9PBG1, |
| UniProt Related Accession: | P48728 |
| Molecular Weight: | 41,780 Da |
| NCBI Full Name: | Aminomethyltransferase, mitochondrial |
| NCBI Synonym Full Names: | aminomethyltransferase |
| NCBI Official Symbol: | AMT |
| NCBI Official Synonym Symbols: | GCE; NKH; GCST; GCVT |
| NCBI Protein Information: | aminomethyltransferase, mitochondrial |
| UniProt Protein Name: | Aminomethyltransferase, mitochondrial |
| UniProt Synonym Protein Names: | Glycine cleavage system T protein |
| Protein Family: | Probable ammonia channel |
| UniProt Gene Name: | AMT |
| UniProt Entry Name: | GCST_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AMT antibody (CAB9926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using AMT Rabbit pAb (CAB9926) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using AMT Rabbit pAb (CAB9926) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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