KO Validated Antibodies 1

Anti-AMPKBeta1 Antibody (CAB12491)[KO Validated]

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SKU:
CAB12491
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-AMPKBeta1 Antibody [KO Validated]
Antibody SKU:CAB12491
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKBeta1 (NP_006244.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, HeLa, Jurkat, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human AMPKBeta1 (NP_006244.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGNT SSER AALE RHGG HKTP RRDS SGGT KDGD RPKI LMDS PEDA DLFH SEEI KAPE KEEF LAWQ HDLE VNDK APAQ ARPT
Gene ID:5564
Uniprot:Q9Y478
Cellular Location:
Calculated MW:30kDa
Observed MW:38kDa
Synonyms:PRKAB1, AMPK, HAMPKb
Background:The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
UniProt Protein Function:AMPKB1: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity. Hematopoietic AMPKB1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity.
UniProt Protein Details:

Protein type:Protein kinase, regulatory subunit; Autophagy

Chromosomal Location of Human Ortholog: 12q24.1-q24.3

Cellular Component: nucleus; cytosol; AMP-activated protein kinase complex

Molecular Function:AMP-activated protein kinase activity; protein binding; protein kinase binding; protein kinase activity

Biological Process: mitochondrion organization and biogenesis; protein heterooligomerization; organelle organization and biogenesis; insulin receptor signaling pathway; cell cycle arrest; signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; fatty acid biosynthetic process

NCBI Summary:The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y478
NCBI GenInfo Identifier:14194425
NCBI Gene ID:5564
NCBI Accession:Q9Y478.4
UniProt Secondary Accession:Q9Y478,Q9UBV0, Q9UE20, Q9UEX2, Q9Y6V8,
UniProt Related Accession:Q9Y478
Molecular Weight:
NCBI Full Name:5'-AMP-activated protein kinase subunit beta-1
NCBI Synonym Full Names:protein kinase, AMP-activated, beta 1 non-catalytic subunit
NCBI Official Symbol:PRKAB1  
NCBI Official Synonym Symbols:AMPK; HAMPKb  
NCBI Protein Information:5'-AMP-activated protein kinase subunit beta-1; AMPKb; AMPK beta 1; AMPK beta -1 chain; AMPK subunit beta-1; AMP-activated protein kinase beta subunit; 5'-AMP-activated protein kinase beta-1 subunit; protein kinase, AMP-activated, noncatalytic, beta-1
UniProt Protein Name:5'-AMP-activated protein kinase subunit beta-1
Protein Family:5'-AMP-activated protein kinase
UniProt Gene Name:PRKAB1  
UniProt Entry Name:AAKB1_HUMAN
Anti-AMPKBeta1 Antibody [KO Validated] (CAB12491)Western blot analysis of extracts of various cell lines, using AMPKb1 antibody (CAB12491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-AMPKBeta1 Antibody [KO Validated] (CAB12491)Western blot analysis of extracts from normal (control) and AMPKb1 knockout (KO) HeLa cells, using AMPKb1 antibody (CAB12491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-AMPKBeta1 Antibody [KO Validated] (CAB12491)Immunohistochemistry of paraffin-embedded human esophagus using AMPKb1 antibody (CAB12491) at dilution of 1:100 (40x lens).
Anti-AMPKBeta1 Antibody [KO Validated] (CAB12491)Immunofluorescence analysis of NIH-3T3 cells using AMPKb1 Polyclonal Antibody (CAB12491) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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