Cell Biology Antibodies 10

Anti-ALPI Antibody (CAB6226)

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SKU:
CAB6226
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ALPI Antibody
Antibody SKU:CAB6226
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human ALPI (NP_001622.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human ALPI (NP_001622.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VIPA EEEN PAFW NRQA AEAL DAAK KLQP IQKV AKNL ILFL GDGL GVPT VTAT RILK GQKN GKLG PETP LAMD RFPY LALS KTYN VDRQ VPDS AATA TAYL CGVK ANFQ TIGL SAAA RFNQ CNTT RGNE VISV MNRA KQAG KSVG VVTT TRVQ HASP AGTY AHTV NRNW YSDA DMPA SARQ EGCQ DIAT QLIS NMDI DVIL GGGR KYMF PMGT PDPE YPAD A
Gene ID:248
Uniprot:P09923
Cellular Location:Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:56kDa
Observed MW:72kDa
Synonyms:ALPI, IAP
Background:There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
UniProt Protein Function:ALPI: intestinal alkaline phosphatase is one of four related alkaline phosphatases. The exact physiological function of alkaline phosphatases is not known. This protein is a GPI-anchored membrane bound glycosylated enzyme that is localized to fetal and adult intestines.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - folate biosynthesis; Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Phosphatase; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37.1

Molecular Function:alkaline phosphatase activity; magnesium ion binding; protease binding; protein binding; zinc ion binding

Biological Process: dephosphorylation

NCBI Summary:There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]
UniProt Code:P09923
NCBI GenInfo Identifier:130744
NCBI Gene ID:248
NCBI Accession:P09923.2
UniProt Secondary Accession:P09923,Q53S80, Q9UBV5, Q9UCL2, B2R7Y4,
UniProt Related Accession:P09923
Molecular Weight:56,812 Da
NCBI Full Name:Intestinal-type alkaline phosphatase
NCBI Synonym Full Names:alkaline phosphatase, intestinal
NCBI Official Symbol:ALPI  
NCBI Official Synonym Symbols:IAP  
NCBI Protein Information:intestinal-type alkaline phosphatase
UniProt Protein Name:Intestinal-type alkaline phosphatase
Protein Family:Intestinal-type alkaline phosphatase
UniProt Gene Name:ALPI  
UniProt Entry Name:PPBI_HUMAN
Anti-ALPI Antibody (CAB6226)Western blot analysis of extracts of HepG2 cells, using ALPI Antibody (CAB6226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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