Metabolism Antibodies 2
Anti-ALOX5 Antibody (CAB2158)
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- SKU:
- CAB2158
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ALOX5 Antibody |
| Antibody SKU: | CAB2158 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ALOX5 (NP_000689.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ALOX5 (NP_000689.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPSY TVTV ATGS QWFA GTDD YIYL SLVG SAGC SEKH LLDK PFYN DFER GAVD SYDV TVDE ELGE IQLV RIEK RKYW LNDD WYLK YITL KTPH GDYI EFPC YRWI TGDV EVVL RDGR AKLA RDDQ IHIL KQHR RKEL ETRQ KQYR WMEW NPGF PLSI DAKC HKDL PRDI QFDS EKGV DFVL NYSK AMEN LFIN RFMH MFQS SWND FADF EKIF VKIS NTIS ERVM NHWQ EDLM FGYQ FLNG CNPV LIRR CTEL PEKL PVTT EMVE CSLE RQLS LEQE VQQG NIFI VDFE LLDG IDAN KTDP |
| Gene ID: | 240 |
| Uniprot: | P09917 |
| Cellular Location: | Cytoplasm, Nucleus matrix, Nucleus membrane, Peripheral membrane protein |
| Calculated MW: | 56kDa/61kDa/71kDa/74kDa/77kDa |
| Observed MW: | 68kDa |
| Synonyms: | ALOX5, 5-LO, 5-LOX, 5LPG, LOG5 |
| Background: | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8, 11, 14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7, 9-trans-11, 14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | 5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - linoleic acid; Nuclear envelope; EC 1.13.11.34; Oxidoreductase; Lipid Metabolism - arachidonic acid Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol Molecular Function:protein binding; iron ion binding; arachidonate 5-lipoxygenase activity Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process Disease: Asthma, Susceptibility To |
| NCBI Summary: | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P09917 |
| NCBI GenInfo Identifier: | 126407 |
| NCBI Gene ID: | 240 |
| NCBI Accession: | P09917.2 |
| UniProt Secondary Accession: | P09917,Q5JQ14, B7ZLS0, E5FPY5, E5FPY7, E5FPY8, |
| UniProt Related Accession: | P09917 |
| Molecular Weight: | 674 |
| NCBI Full Name: | Arachidonate 5-lipoxygenase |
| NCBI Synonym Full Names: | arachidonate 5-lipoxygenase |
| NCBI Official Symbol: | ALOX5 |
| NCBI Official Synonym Symbols: | 5-LO; 5LPG; LOG5; 5-LOX |
| NCBI Protein Information: | arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform |
| UniProt Protein Name: | Arachidonate 5-lipoxygenase |
| Protein Family: | Arachidonate 5-lipoxygenase |
| UniProt Gene Name: | ALOX5 |
| UniProt Entry Name: | LOX5_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using ALOX5 antibody (CAB2158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human tonsil using ALOX5 antibody (CAB2158) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using ALOX5 antibody (CAB2158) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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