Metabolism Antibodies 2

Anti-ALOX15 Antibody (CAB6864)

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SKU:
CAB6864
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-ALOX15 Antibody (CAB6864)
Antibody SKU:CAB6864
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALOX15 (NP_001131.3).
Application:Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:COS-7, Mouse stomach, Mouse skin, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALOX15 (NP_001131.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGLY RIRV STGA SLYA GSNN QVQL WLVG QHGE AALG KRLW PARG KETE LKVE VPEY LGPL LFVK LRKR HLLK DDAW FCNW ISVQ GPGA GDEV RFPC YRWV EGNG VLSL PEGT GRTV GEDP QGLF QKHR EEEL EERR KLYR WGNW KDGL ILNM AGAK LYDL PVDE RFLE DKRV DFEV SLAK GLAD LAIK DSLN VLTC WKDL DDFN RIFW CGQS KLAE RVRD SWKE DALF GY
Cellular Location:Cell membrane, Cytoplasm, Lipid droplet, Peripheral membrane protein, cytosol
Calculated MW:70kDa/74kDa
Observed MW:65kDa
Synonyms:ALOX15, 12-LOX, 15-LOX-1, 15LOX-1
Background:0
Immunohistochemistry of paraffin-embedded rat lung using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat lung using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human stomach cancer using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human stomach cancer using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using ALOX15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Western blot analysis of extracts of various cell lines, using ALOX15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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