Description
| Antibody Name: | Anti-ALOX15 Antibody (CAB6864) |
| Antibody SKU: | CAB6864 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALOX15 (NP_001131.3). |
| Application: | Western blotting, Immunohistochemistry, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | COS-7, Mouse stomach, Mouse skin, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALOX15 (NP_001131.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGLY RIRV STGA SLYA GSNN QVQL WLVG QHGE AALG KRLW PARG KETE LKVE VPEY LGPL LFVK LRKR HLLK DDAW FCNW ISVQ GPGA GDEV RFPC YRWV EGNG VLSL PEGT GRTV GEDP QGLF QKHR EEEL EERR KLYR WGNW KDGL ILNM AGAK LYDL PVDE RFLE DKRV DFEV SLAK GLAD LAIK DSLN VLTC WKDL DDFN RIFW CGQS KLAE RVRD SWKE DALF GY |
| Cellular Location: | Cell membrane, Cytoplasm, Lipid droplet, Peripheral membrane protein, cytosol |
| Calculated MW: | 70kDa/74kDa |
| Observed MW: | 65kDa |
| Synonyms: | ALOX15, 12-LOX, 15-LOX-1, 15LOX-1 |
| Background: | 0 |
 | Immunohistochemistry of paraffin-embedded rat lung using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human stomach cancer using ALOX15 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of various cell lines, using ALOX15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
