Metabolism Antibodies 3
Anti-ALOX12B Antibody (CAB16210)
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- SKU:
- CAB16210
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ALOX12B Antibody |
| Antibody SKU: | CAB16210 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALOX12B (NP_001130.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, Mouse spleen, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALOX12B (NP_001130.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATY KVRV ATGT DLLS GTRD SISL TIVG TQGE SHKQ LLNH FGRD FATG AVGQ YTVQ CPQD LGEL IIIR LHKE RYAF FPKD PWYC NYVQ ICAP NGRI YHFP AYQW MDGY ETLA LREA TGKT TADD SLPV LLEH RKEE IRAK QDFY HWRV FLPG LPSY VHIP SYRP PVRR HRNP NRPE WNGY IPGF PILI NFKA TKFL NLNL RYSF LKTA SFFV RLGP MALA FKVR GLLD CKHS WKRL KDIR KIFP GKKS VV |
| Gene ID: | 242 |
| Uniprot: | O75342 |
| Cellular Location: | |
| Calculated MW: | 80kDa |
| Observed MW: | 80kDa |
| Synonyms: | ALOX12B, 12R-LOX, ARCI2, 12R-type |
| Background: | This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. |
| UniProt Code: | O75342 |
| NCBI GenInfo Identifier: | 4502053 |
| NCBI Gene ID: | 242 |
| NCBI Accession: | NP_001130.1 |
| UniProt Related Accession: | O75342 |
| Molecular Weight: | 80,356 Da |
| NCBI Full Name: | arachidonate 12-lipoxygenase, 12R-type |
| NCBI Synonym Full Names: | arachidonate 12-lipoxygenase, 12R type |
| NCBI Official Symbol: | ALOX12B |
| NCBI Official Synonym Symbols: | ARCI2; 12R-LOX |
| NCBI Protein Information: | arachidonate 12-lipoxygenase, 12R-type; 12R-lipoxygenase; epidermis-type lipoxygenase 12 |
| UniProt Protein Name: | Arachidonate 12-lipoxygenase, 12R-type |
| UniProt Synonym Protein Names: | Epidermis-type lipoxygenase 12 |
| Protein Family: | Arachidonate 12-lipoxygenase |
| UniProt Gene Name: | ALOX12B |
| UniProt Entry Name: | LX12B_HUMAN |
 | Western blot analysis of extracts of Rat testis, using ALOX12B antibody (CAB16210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of extracts of various cell lines, using ALOX12B antibody (CAB16210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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