Epigenetics & Nuclear Signaling Antibodies 4

Anti-ALKBH4 Antibody (CAB7812)

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SKU:
CAB7812
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ALKBH4 Antibody
Antibody SKU:CAB7812
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ALKBH4 (NP_060091.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human
Positive Samples:SKOV3, NCI-H125, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ALKBH4 (NP_060091.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAA AAET PEVL RECG CKGI RTCL ICER QRGS DPPW ELPP AKTY RFIY CSDT GWAV GTEE SDFE GWAF PFPG VMLI EDFV TREE EAEL VRLM DRDP WKLS QSGR RKQD YGPK VNFR KQKL KTEG FCGL PSFS REVV RRMG LYPG LEGF RPVE QCNL DYCP ERGS AIDP HL
Gene ID:54784
Uniprot:Q9NXW9
Cellular Location:Cytoplasm, Midbody, Nucleus
Calculated MW:4kDa/33kDa
Observed MW:36kDa
Synonyms:ALKBH4, ABH4
Background:
UniProt Protein Function:ALKBH4: Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.11.-

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cytoplasm; midbody; nucleus

Molecular Function:metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; actin binding; demethylase activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; actomyosin structure organization and biogenesis; protein amino acid demethylation

UniProt Code:Q9NXW9
NCBI GenInfo Identifier:74734701
NCBI Gene ID:54784
NCBI Accession:Q9NXW9.1
UniProt Related Accession:Q9NXW9
Molecular Weight:
NCBI Full Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
NCBI Synonym Full Names:alkB homolog 4, lysine demethylase
NCBI Official Symbol:ALKBH4  
NCBI Official Synonym Symbols:ABH4  
NCBI Protein Information:alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
UniProt Protein Name:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
UniProt Synonym Protein Names:Alkylated DNA repair protein alkB homolog 4
UniProt Gene Name:ALKBH4  
UniProt Entry Name:ALKB4_HUMAN
Anti-ALKBH4 Antibody (CAB7812)Western blot analysis of extracts of various cell lines, using ALKBH4 Antibody (CAB7812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-ALKBH4 Antibody (CAB7812)Immunohistochemistry of paraffin-embedded human stomach using ALKBH4 antibody (CAB7812) at dilution of 1:100 (40x lens).
Anti-ALKBH4 Antibody (CAB7812)Immunohistochemistry of paraffin-embedded human liver damage using ALKBH4 antibody (CAB7812) at dilution of 1:100 (40x lens).
Anti-ALKBH4 Antibody (CAB7812)Immunohistochemistry of paraffin-embedded human lung cancer using ALKBH4 antibody (CAB7812) at dilution of 1:100 (40x lens).
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