Name: Anti-ALKBH1 Antibody (CAB14079)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB14079
Availability: OutOfStock

Antibody Name:	Anti-ALKBH1 Antibody
Antibody SKU:	CAB14079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YQWH WVKQ CLKL YSQK PNVC NLDK HMSK EETQ DLWE QSKE FLRY KEAT KRRP RSLL EKLR WVTV GYHY NWDS KKYS ADHY TPFP SDLG FLSE QVAA ACGF EDFR AEAG ILNY YRLD STLG IHVD RSEL DHSK PLLS FSFG QSAI FLLG GLQR DEAP TAMF MHSG DIMI MSGF SRLL NHAV PRVL PNPE GEGL PHCL EAPL PAVL PRDS MVEP CSME DWQV CASY LKTA RVNM TVRQ VLAT DQNF PLEP IEDE KRDI STEG FCHL DDQN SEVK RARI NPDS
Gene ID:	8846
Uniprot:	Q13686
Cellular Location:	Mitochondrion, Nucleus
Calculated MW:	43kDa
Observed MW:	44kDa

Synonyms:	ALKBH1, ABH, ABH1, ALKBH, alkB, hABH
Background:	This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

UniProt Protein Function:	ALKBH: Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. Belongs to the alkB family.
UniProt Protein Details:	Protein type:DNA repair, damage; EC 4.2.99.18; EC 1.14.11.33 Chromosomal Location of Human Ortholog: 14q24.3 Cellular Component: mitochondrion Molecular Function:DNA-(apurinic or apyrimidinic site) lyase activity; ferrous iron binding; chemoattractant activity Biological Process: developmental growth; DNA dealkylation; positive chemotaxis; in utero embryonic development; neuron migration; RNA repair; negative regulation of neuron apoptosis; DNA repair; DNA catabolic process, endonucleolytic; neurite development; placenta development
NCBI Summary:	This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13686
NCBI GenInfo Identifier:	12643239
NCBI Gene ID:	8846
NCBI Accession:	Q13686.2
UniProt Secondary Accession:	Q13686,Q8TAU1, Q9ULA7,
UniProt Related Accession:	Q13686
Molecular Weight:	389
NCBI Full Name:	Alkylated DNA repair protein alkB homolog 1
NCBI Synonym Full Names:	alkB, alkylation repair homolog 1 (E. coli)
NCBI Official Symbol:	ALKBH1
NCBI Official Synonym Symbols:	ABH; ABH1; alkB; hABH; ALKBH
NCBI Protein Information:	alkylated DNA repair protein alkB homolog 1; DNA lyase ABH1; alkylation repair, alkB homolog; DNA oxidative demethylase ALKBH1; alpha-ketoglutarate-dependent dioxygenase ABH1
UniProt Protein Name:	Alkylated DNA repair protein alkB homolog 1
UniProt Synonym Protein Names:	Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1 (EC:4.2.99.18); DNA oxidative demethylase ALKBH1
Protein Family:	DNA demethylase
UniProt Gene Name:	ALKBH1
UniProt Entry Name:	ALKB1_HUMAN

	Western blot analysis of extracts of 293T cells, using ALKBH1 antibody (CAB14079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using ALKBH1 antibody (CAB14079). Blue: DAPI for nuclear staining.