Cell Death Antibodies 1

Anti-ALIX / PDCD6IP Antibody (CAB2215)

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SKU:
CAB2215
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-ALIX / PDCD6IP Antibody
Antibody SKU:CAB2215
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALIX / PDCD6IP (NP_037506.2).
Application:WB IHC IF
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, LO2, Jurkat, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALIX / PDCD6IP (NP_037506.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATF ISVQ LKKT SEVD LAKP LVKF IQQT YPSG GEEQ AQYC RAAE ELSK LRRA AVGR PLDK HEGA LETL LRYY DQIC SIEP KFPF SENQ ICLT FTWK DAFD KGSL FGGS VKLA LASL GYEK SCVL FNCA ALAS QIAA EQNL DNDE GLKI AAKH YQFA SGAF LHIK ETVL SALS REPT VDIS
Gene ID:10015
Uniprot:Q8WUM4
Cellular Location:Cytoplasm, Melanosome, centrosome, cytoskeleton, cytosol, microtubule organizing center
Calculated MW:30kDa/96kDa
Observed MW:105kDa
Synonyms:PDCD6IP, AIP1, ALIX, DRIP4, HP95
Background:This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
UniProt Protein Function:Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.
UniProt Protein Details:

Protein type:Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle

Molecular Function:protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding

Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis

NCBI Summary:This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]
UniProt Code:Q8WUM4
NCBI GenInfo Identifier:31076831
NCBI Gene ID:10015
NCBI Accession:Q8WUM4.1
UniProt Secondary Accession:Q8WUM4,Q6NUS1, Q9BX86, Q9NUN0, Q9P2H2, Q9UKL5, C5MQH7 E9PFU1,
UniProt Related Accession:Q8WUM4
Molecular Weight:868
NCBI Full Name:Programmed cell death 6-interacting protein
NCBI Synonym Full Names:programmed cell death 6 interacting protein
NCBI Official Symbol:PDCD6IP  
NCBI Official Synonym Symbols:AIP1; ALIX; HP95; DRIP4  
NCBI Protein Information:programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; dopamine receptor interacting protein 4; apoptosis-linked gene 2-interacting protein X
UniProt Protein Name:Programmed cell death 6-interacting protein
UniProt Synonym Protein Names:ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95
Protein Family:Programmed cell death 6-interacting protein
UniProt Gene Name:PDCD6IP  
UniProt Entry Name:PDC6I_HUMAN
Anti-ALIX / PDCD6IP Antibody (CAB2215)Western blot analysis of extracts of various cell lines, using ALIX / PDCD6IP antibody (CAB2215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-ALIX / PDCD6IP Antibody (CAB2215)Immunohistochemistry of paraffin-embedded rat kidney using ALIX / PDCD6IP antibody (CAB2215) at dilution of 1:100 (40x lens).
Anti-ALIX / PDCD6IP Antibody (CAB2215)Immunohistochemistry of paraffin-embedded human prostate using ALIX / PDCD6IP antibody (CAB2215) at dilution of 1:100 (40x lens).
Anti-ALIX / PDCD6IP Antibody (CAB2215)Immunohistochemistry of paraffin-embedded human stomach using ALIX / PDCD6IP antibody (CAB2215) at dilution of 1:100 (40x lens).
Anti-ALIX / PDCD6IP Antibody (CAB2215)Immunofluorescence analysis of L929 cells using ALIX / PDCD6IP antibody (CAB2215) at dilution of 1:100. Blue: DAPI for nuclear staining.
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