Metabolism Antibodies 2

Anti-ALDOB Antibody (CAB3728)

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SKU:
CAB3728
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ALDOB Antibody
Antibody SKU:CAB3728
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALDOB (NP_000026.2).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, K-562, MCF7, Mouse liver, Mouse kidney, Mouse skeletal muscle, Rat liver, Rat kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALDOB (NP_000026.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAHR FPAL TQEQ KKEL SEIA QSIV ANGK GILA ADES VGTM GNRL QRIK VENT EENR RQFR EILF SVDS SINQ SIGG VILF HETL YQKD SQGK LFRN ILKE KGIV VGIK LDQG GAPL AGTN KETT IQGL DGLS ERCA QYKK DGVD FGKW RAVL RIAD QCPS SLAI QENA NALA RYAS ICQQ NGLV PIVE PEVI PDGD HDLE
Gene ID:229
Uniprot:P05062
Cellular Location:Cytoplasm, centriolar satellite, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:39kDa
Observed MW:39kDa
Synonyms:ALDOB, ALDB, ALDO2
Background:Fructose-1, 6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.
UniProt Protein Function:ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 9q21.3-q22.2

Cellular Component: microtubule organizing center; cytosol

Molecular Function:identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; ATPase binding

Biological Process: fructose 1,6-bisphosphate metabolic process; NADH oxidation; glycolysis; positive regulation of ATPase activity; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose catabolic process; gluconeogenesis; fructose metabolic process

Disease: Fructose Intolerance, Hereditary

NCBI Summary:Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]
UniProt Code:P05062
NCBI GenInfo Identifier:113611
NCBI Gene ID:229
NCBI Accession:P05062.2
UniProt Secondary Accession:P05062,Q13741, Q13742, Q5T7D6,
UniProt Related Accession:P05062
Molecular Weight:Calculated MW: 39kDaObserved MW: 39kDa
NCBI Full Name:Fructose-bisphosphate aldolase B
NCBI Synonym Full Names:aldolase B, fructose-bisphosphate
NCBI Official Symbol:ALDOB  
NCBI Official Synonym Symbols:ALDB; ALDO2  
NCBI Protein Information:fructose-bisphosphate aldolase B; aldolase 2; liver-type aldolase; aldolase B, fructose-bisphosphatase
UniProt Protein Name:Fructose-bisphosphate aldolase B
UniProt Synonym Protein Names:Liver-type aldolase
Protein Family:Fructose-bisphosphate aldolase
UniProt Gene Name:ALDOB  
UniProt Entry Name:ALDOB_HUMAN
Anti-ALDOB Antibody (CAB3728)Western blot analysis of extracts of various cell lines, using ALDOB antibody (CAB3728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ALDOB Antibody (CAB3728)Immunofluorescence analysis of NIH/3T3 cells using ALDOB Rabbit pAb (CAB3728) at dilution of 1:100. Blue: DAPI for nuclear staining.
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