Metabolism Antibodies 1

Anti-ALDOA Antibody (CAB1142)

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SKU:
CAB1142
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ALDOA Antibody
Antibody SKU:CAB1142
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ALDOA (NP_908930.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:20 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, DU145, 293T, HepG2, Jurkat, A-549, CEM, Mouse skeletal muscle, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-348 of human ALDOA (NP_908930.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPYQ YPAL TPEQ KKEL SDIA HRIV APGK GILA ADES TGSI AKRL QSIG TENT EENR RFYR QLLL TADD RVNP CIGG VILF HETL YQKA DDGR PFPQ VIKS KGGV VGIK VDKG VVPL AGTN GETT TQGL DGLS ERCA QYKK DGAD FAKW RCVL KIGE HTPS ALAI MENA NVLA RYAS ICQQ NGIV PIVE PEIL PDGD HDLK RCQY VTEK VLAA VYKA LSDH HIYL EGTL LKPN MVTP GHAC TQKF SHEE IAMA TVTA LRRT VPPA VTGI TFLS GGQS EEEA SINL NAIN KCPL LKPW ALTF SYGR ALQA SALK AWGG KKEN LKAA QEEY VKRA LANS LACQ GKYT PSGQ
Gene ID:226
Uniprot:P04075
Cellular Location:Cytoplasm, I band, M line, myofibril, sarcomere
Calculated MW:39kDa/45kDa
Observed MW:43kDa
Synonyms:ALDOA, ALDA, GSD12, HEL-S-87p
Background:The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.
UniProt Protein Function:ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Carbohydrate Metabolism - fructose and mannose; Lyase; Carbohydrate Metabolism - pentose phosphate pathway; Mitochondrial

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton

Molecular Function:tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding

Biological Process: platelet activation; striated muscle contraction; glycolysis; glucose metabolic process; actin filament organization; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process

Disease: Glycogen Storage Disease Xii

NCBI Summary:The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]
UniProt Code:P04075
NCBI GenInfo Identifier:113606
NCBI Gene ID:226
NCBI Accession:P04075.2
UniProt Secondary Accession:P04075,Q6FH76, Q6FI10, Q96B15, Q9BWD9, Q9UCN2, B4DXI7
UniProt Related Accession:P04075
Molecular Weight:364
NCBI Full Name:Fructose-bisphosphate aldolase A
NCBI Synonym Full Names:aldolase A, fructose-bisphosphate
NCBI Official Symbol:ALDOA  
NCBI Official Synonym Symbols:ALDA; GSD12; HEL-S-87p  
NCBI Protein Information:fructose-bisphosphate aldolase A; muscle-type aldolase; lung cancer antigen NY-LU-1; fructose-1,6-bisphosphate triosephosphate-lyase; epididymis secretory sperm binding protein Li 87p
UniProt Protein Name:Fructose-bisphosphate aldolase A
UniProt Synonym Protein Names:Lung cancer antigen NY-LU-1; Muscle-type aldolase
Protein Family:Fructose-bisphosphate aldolase
UniProt Gene Name:ALDOA  
UniProt Entry Name:ALDOA_HUMAN
Anti-ALDOA Antibody (CAB1142)Western blot analysis of extracts of various cell lines, using ALDOA antibody (CAB1142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
Anti-ALDOA Antibody (CAB1142)Immunofluorescence analysis of A549 cells using ALDOA antibody (CAB1142). Blue: DAPI for nuclear staining.
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