Antibody Name:	Anti-ALDH7A1 Antibody
Antibody SKU:	CAB8629
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-539 of human ALDH7A1 (NP_001173.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, SKOV3, 293T, HeLa, Mouse liver, Mouse kidney, Rat liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 430-539 of human ALDH7A1 (NP_001173.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APIL YVFK FKNE EEVF AWNN EVKQ GLSS SIFT KDLG RIFR WLGP KGSD CGIV NVNI PTSG AEIG GAFG GEKH TGGG RESG SDAW KQYM RRST CTIN YSKD LPLA QGIK FQ
Gene ID:	501
Uniprot:	P49419
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, cytosol
Calculated MW:	51kDa/55kDa/58kDa
Observed MW:	58kDa

Synonyms:

ALDH7A1, ATQ1, EPD, PDE

Background:

The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified.

UniProt Protein Function:	ALDH7A1: Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism. Defects in ALDH7A1 are the cause of pyridoxine-dependent epilepsy (PDE). PDE is characterized by a combination of various seizure types. It usually occurs in the first hours of life and is unresponsive to standard anticonvulsants, responding only to immediate administration of pyridoxine hydrochloride. Belongs to the aldehyde dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.2.1.3; EC 1.2.1.31; EC 1.2.1.8; Lipid Metabolism - fatty acid; Lipid Metabolism - glycerolipid; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Secondary Metabolites Metabolism - limonene and pinene degradation Chromosomal Location of Human Ortholog: 5q31 Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion Molecular Function:aldehyde dehydrogenase (NAD) activity; betaine-aldehyde dehydrogenase activity; L-aminoadipate-semialdehyde dehydrogenase activity; protein binding Biological Process: aldehyde metabolic process; choline catabolic process; lysine catabolic process; sensory perception of sound Disease: Epilepsy, Pyridoxine-dependent
NCBI Summary:	The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified. [provided by RefSeq, Jan 2011]
UniProt Code:	P49419
NCBI GenInfo Identifier:	982742419
NCBI Gene ID:	501
NCBI Accession:
UniProt Secondary Accession:	P49419,O14619, Q6IPU8, Q9BUL4, B2R669, B4DIC7, B4DMA0 E7EPT3,
UniProt Related Accession:	P49419
Molecular Weight:	51,411 Da
NCBI Full Name:	alpha-aminoadipic semialdehyde dehydrogenase isoform 3
NCBI Synonym Full Names:	aldehyde dehydrogenase 7 family member A1
NCBI Official Symbol:	ALDH7A1
NCBI Official Synonym Symbols:	EPD; PDE; ATQ1
NCBI Protein Information:	alpha-aminoadipic semialdehyde dehydrogenase
UniProt Protein Name:	Alpha-aminoadipic semialdehyde dehydrogenase
UniProt Synonym Protein Names:	Aldehyde dehydrogenase family 7 member A1 (EC:1.2.1.3); Antiquitin-1; Betaine aldehyde dehydrogenase (EC:1.2.1.8); Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase
Protein Family:	Alpha-aminoadipic semialdehyde dehydrogenase
UniProt Gene Name:	ALDH7A1
UniProt Entry Name:	AL7A1_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH7A1 antibody (CAB8629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using ALDH7A1 antibody (CAB8629) at dilution of 1:100. Blue: DAPI for nuclear staining.