Name: Anti-ALDH1L1 Antibody (CAB7067)
Brand: Metabolism Antibodies 2
SKU: CAB7067
Availability: OutOfStock

Antibody Name:	Anti-ALDH1L1 Antibody
Antibody SKU:	CAB7067
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALDH1L1 (NP_036322.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, MCF7, Jurkat, Mouse liver, Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALDH1L1 (NP_036322.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKIA VIGQ SLFG QEVY CHLR KEGH EVVG VFTV PDKD GKAD PLGL EAEK DGVP VFKY SRWR AKGQ ALPD VVAK YQAL GAEL NVLP FCSQ FIPM EIIS APRH GSII YHPS LLPR HRGA SAIN WTLI HGDK KGGF SIFW ADDG LDTG DLLL QKEC EVLP DDTV STLY NRFL FPEG IKGM VQAV RLIA EGKA PRLP QPEE GATY EGIQ KKET AKIN WDQP AEAI HNWI RGND KVPG AWTE ACEQ KLTF FNST LN
Gene ID:	10840
Uniprot:	O75891
Cellular Location:	Cytoplasm
Calculated MW:	55kDa/87kDa/98kDa/99kDa
Observed MW:	120kDa

Synonyms:

ALDH1L1, 10-FTHFDH, 10-fTHF, FDH, FTHFD

Background:

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ALDH1L1: 2 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.6 Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: mitochondrion Molecular Function:methyltransferase activity; aldehyde dehydrogenase (NAD) activity; formyltetrahydrofolate dehydrogenase activity; hydroxymethyl-, formyl- and related transferase activity; catalytic activity Biological Process: methylation; biosynthetic process; one-carbon compound metabolic process; 10-formyltetrahydrofolate catabolic process
NCBI Summary:	The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
UniProt Code:	O75891
NCBI GenInfo Identifier:	59802911
NCBI Gene ID:	10840
NCBI Accession:	O75891.2
UniProt Secondary Accession:	O75891,Q68CS1, B4DG36, E9PBX3,
UniProt Related Accession:	O75891
Molecular Weight:	902
NCBI Full Name:	Cytosolic 10-formyltetrahydrofolate dehydrogenase
NCBI Synonym Full Names:	aldehyde dehydrogenase 1 family, member L1
NCBI Official Symbol:	ALDH1L1
NCBI Official Synonym Symbols:	FDH; FTHFD; 10-fTHF; 10-FTHFDH
NCBI Protein Information:	cytosolic 10-formyltetrahydrofolate dehydrogenase; MER57B-ALDH1L1
UniProt Protein Name:	Cytosolic 10-formyltetrahydrofolate dehydrogenase
UniProt Synonym Protein Names:	Aldehyde dehydrogenase family 1 member L1
Protein Family:	Cytosolic 10-formyltetrahydrofolate dehydrogenase
UniProt Gene Name:	ALDH1L1
UniProt Entry Name:	AL1L1_HUMAN

Western blot analysis of extracts of various cell lines, using ALDH1L1 antibody (CAB7067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

Anti-ALDH1L1 Antibody (CAB7707)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)