Name: Anti-ALDH1B1 Antibody (CAB3725)
Brand: Cell Biology Antibodies 8
SKU: CAB3725
Availability: OutOfStock

Antibody Name:	Anti-ALDH1B1 Antibody
Antibody SKU:	CAB3725
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-517 of human ALDH1B1 (NP_000683.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, HepG2, SKOV3, Mouse testis, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 278-517 of human ALDH1B1 (NP_000683.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLKR VTLE LGGK SPSI VLAD ADME HAVE QCHE ALFF NMGQ CCCA GSRT FVEE SIYN EFLE RTVE KAKQ RKVG NPFE LDTQ QGPQ VDKE QFER VLGY IQLG QKEG AKLL CGGE RFGE RGFF IKPT VFGG VQDD MRIA KEEI FGPV QPLF KFKK IEEV VERA NNTR YGLA AAVF TRDL DKAM YFTQ ALQA GTVW VNTY NIVT CHTP FGGF KESG NGRE LGED GLKA YTEV KTVT IKVP QKNS
Gene ID:	219
Uniprot:	P30837
Cellular Location:	Mitochondrion matrix
Calculated MW:	57kDa
Observed MW:	57kDa

Synonyms:	ALDH1B1, ALDH5, ALDHX
Background:	This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems.

UniProt Protein Function:	ALDH1B1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. Belongs to the aldehyde dehydrogenase family.
UniProt Protein Details:	Protein type:Oxidoreductase; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - lysine degradation; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; EC 1.2.1.3; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - propanoate Chromosomal Location of Human Ortholog: 9p11.1 Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; mitochondrial matrix Molecular Function:aldehyde dehydrogenase (NAD) activity Biological Process: ethanol catabolic process; carbohydrate metabolic process
NCBI Summary:	This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq, Jul 2008]
UniProt Code:	P30837
NCBI GenInfo Identifier:	311033472
NCBI Gene ID:	219
NCBI Accession:	P30837.3
UniProt Secondary Accession:	P30837,Q8WX76, Q9BV45, B2R8F0,
UniProt Related Accession:	P30837
Molecular Weight:	517
NCBI Full Name:	Aldehyde dehydrogenase X, mitochondrial
NCBI Synonym Full Names:	aldehyde dehydrogenase 1 family, member B1
NCBI Official Symbol:	ALDH1B1
NCBI Official Synonym Symbols:	ALDH5; ALDHX
NCBI Protein Information:	aldehyde dehydrogenase X, mitochondrial; ALDH class 2; aldehyde dehydrogenase 5; acetaldehyde dehydrogenase 5
UniProt Protein Name:	Aldehyde dehydrogenase X, mitochondrial
UniProt Synonym Protein Names:	Aldehyde dehydrogenase 5; Aldehyde dehydrogenase family 1 member B1
Protein Family:	Aldehyde dehydrogenase
UniProt Gene Name:	ALDH1B1
UniProt Entry Name:	AL1B1_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH1B1 antibody (CAB3725) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using ALDH1B1 Rabbit pAb (CAB3725) at dilution of 1:100. Blue: DAPI for nuclear staining.