Antibody Name:	Anti-ALDH1A2 Antibody
Antibody SKU:	CAB7503
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, 293T, Mouse liver, Mouse kidney, Mouse testis, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH1A2 (NP_733797.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTSS KIEM PGEV KADP AALM ASLH LLPS PTPN LEIK YTKI FINN EWQN SESG RVFP VYNP ATGE QVCE VQEA DKAD IDKA VQAA RLAF SLGS VWRR MDAS ERGR LLDK LADL VERD RAVL ATME SLNG GKPF LQAF YVDL QGVI KTFR YYAG WADK IHGM TIPV DGDY FTFT RHEP IGVC
Gene ID:	8854
Uniprot:	O94788
Cellular Location:	Cytoplasm
Calculated MW:	46kDa/53kDa/54kDa/56kDa
Observed MW:	57kDa

Synonyms:

ALDH1A2, RALDH(II), RALDH2, RALDH2-T

Background:

This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	ALDH1A2: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.2.1.36; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase Chromosomal Location of Human Ortholog: 15q21.3 Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol Molecular Function:aldehyde dehydrogenase (NAD) activity; 3-chloroallyl aldehyde dehydrogenase activity; retinal binding; retinal dehydrogenase activity Biological Process: neural tube development; embryonic forelimb morphogenesis; retinoic acid receptor signaling pathway; heart morphogenesis; retinal metabolic process; neural crest cell development; positive regulation of apoptosis; vitamin A metabolic process; response to estradiol stimulus; response to vitamin A; anterior/posterior pattern formation; neuron differentiation; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; retinol metabolic process; pancreas development; determination of bilateral symmetry; positive regulation of cell proliferation; retinoic acid metabolic process; kidney development; embryonic gut development; proximal/distal pattern formation; hindbrain development; cardiac muscle development; blood vessel development; regulation of endothelial cell proliferation; liver development; embryonic camera-type eye development; pituitary gland development; response to cytokine stimulus; 9-cis-retinoic acid biosynthetic process; midgut development; lung development
NCBI Summary:	This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011]
UniProt Code:	O94788
NCBI GenInfo Identifier:	90109797
NCBI Gene ID:	8854
NCBI Accession:	O94788.3
UniProt Secondary Accession:	O94788,Q2PJS6, Q8NHQ4, Q9UBR8, Q9UFY0, B3KY52, B4DZR2 F5H2Y9, H0YM00,
UniProt Related Accession:	O94788
Molecular Weight:	480
NCBI Full Name:	Retinal dehydrogenase 2
NCBI Synonym Full Names:	aldehyde dehydrogenase 1 family, member A2
NCBI Official Symbol:	ALDH1A2
NCBI Official Synonym Symbols:	RALDH2; RALDH2-T; RALDH(II)
NCBI Protein Information:	retinal dehydrogenase 2; RALDH 2; retinaldehyde-specific dehydrogenase type 2
UniProt Protein Name:	Retinal dehydrogenase 2
UniProt Synonym Protein Names:	Aldehyde dehydrogenase family 1 member A2; Retinaldehyde-specific dehydrogenase type 2; RALDH(II)
Protein Family:	Retinal dehydrogenase
UniProt Gene Name:	ALDH1A2
UniProt Entry Name:	AL1A2_HUMAN

	Western blot analysis of extracts of various cell lines, using ALDH1A2 antibody (CAB7503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A2 antibody (CAB7503) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using ALDH1A2 antibody (CAB7503) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using ALDH1A2 antibody (CAB7503) at dilution of 1:100. Blue: DAPI for nuclear staining.