Cell Biology Antibodies 7

Anti-ALDH1A1 Antibody (CAB1802)

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SKU:
CAB1802
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ALDH1A1 Antibody
Antibody SKU:CAB1802
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HT-29, K-562, Mouse liver, Mouse lung, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELCQ VEEG DKED VDKA VKAA RQAF QIGS PWRT MDAS ERGR LLYK LADL IERD RLLL ATME SMNG GKLY SNAY LNDL AGCI KTLR YCAG WADK IQGR TIPI
Gene ID:216
Uniprot:P00352
Cellular Location:Cytoplasm
Calculated MW:54kDa
Observed MW:50-55kDa
Synonyms:ALDH1A1, ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1
Background:The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
UniProt Protein Function:ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
UniProt Protein Details:

Protein type:GAPs, Ras; EC 1.2.1.36; GAPs; Cofactor and Vitamin Metabolism - retinol; Apoptosis; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function:aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation

NCBI Summary:The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
UniProt Code:P00352
NCBI GenInfo Identifier:118495
NCBI Gene ID:216
NCBI Accession:P00352.2
UniProt Related Accession:P00352
Molecular Weight:~ 50-55kDa
NCBI Full Name:Retinal dehydrogenase 1
NCBI Synonym Full Names:aldehyde dehydrogenase 1 family member A1
NCBI Official Symbol:ALDH1A1  
NCBI Official Synonym Symbols:ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e  
NCBI Protein Information:retinal dehydrogenase 1
UniProt Protein Name:Retinal dehydrogenase 1
UniProt Synonym Protein Names:ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic
Protein Family:Retinal dehydrogenase
UniProt Gene Name:ALDH1A1  
UniProt Entry Name:AL1A1_HUMAN
Anti-ALDH1A1 Antibody (CAB1802)Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (CAB1802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Anti-ALDH1A1 Antibody (CAB1802)Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (CAB1802) at dilution of 1:100 (40x lens).
Anti-ALDH1A1 Antibody (CAB1802)Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (CAB1802) at dilution of 1:100 (40x lens).
Anti-ALDH1A1 Antibody (CAB1802)Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (CAB1802) at dilution of 1:100 (40x lens).
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