Metabolism Antibodies 2

Anti-ALAS1 Antibody (CAB6521)

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SKU:
CAB6521
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ALAS1 Antibody
Antibody SKU:CAB6521
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALAS1 (NP_954635.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, THP-1, SW620, HepG2, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALAS1 (NP_954635.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MESV VRRC PFLS RVPQ AFLQ KAGK SLLF YAQN CPKM MEVG AKPA PRAL STAA VHYQ QIKE TPPA SEKD KTAK AKVQ QTPD GSQQ SPDG TQLP SGHP LPAT SQGT ASKC PFLA AQMN QRGS SVFC KASL ELQE DVQE MNAV RKEV AETS AGPS VVSV KTDG GDPS GLLK NFQD IMQK QRPE RVSH LLQD NLPK SVST FQYD RFFE KKID EKKN DHTY RVFK TVNR RAHI FPMA DDYS DSLI TKKQ VSVW CS
Gene ID:211
Uniprot:P13196
Cellular Location:Mitochondrion matrix
Calculated MW:12kDa/70kDa
Observed MW:70kDa
Synonyms:ALAS1, ALAS, ALAS-H, ALAS3, ALASH, MIG4
Background:This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:ALAS1: the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
UniProt Protein Details:

Protein type:EC 2.3.1.37; Amino Acid Metabolism - glycine, serine and threonine; Mitochondrial; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm

Molecular Function:5-aminolevulinate synthase activity; pyridoxal phosphate binding

Biological Process: mitochondrion organization and biogenesis; organelle organization and biogenesis; porphyrin metabolic process; cellular lipid metabolic process; protoporphyrinogen IX biosynthetic process; heme biosynthetic process

NCBI Summary:This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
UniProt Code:P13196
NCBI GenInfo Identifier:122824
NCBI Gene ID:211
NCBI Accession:P13196.2
UniProt Related Accession:P13196
Molecular Weight:640
NCBI Full Name:5-aminolevulinate synthase, nonspecific, mitochondrial
NCBI Synonym Full Names:5'-aminolevulinate synthase 1
NCBI Official Symbol:ALAS1  
NCBI Official Synonym Symbols:ALAS; MIG4; ALAS3; ALASH  
NCBI Protein Information:5-aminolevulinate synthase, nonspecific, mitochondrial; ALAS-H; delta-ALA synthase 1; migration-inducing protein 4; 5-aminolevulinic acid synthase 1; delta-aminolevulinate synthase 1; aminolevulinate, delta-, synthase 1
UniProt Protein Name:5-aminolevulinate synthase, nonspecific, mitochondrial
UniProt Synonym Protein Names:5-aminolevulinic acid synthase 1; Delta-ALA synthase 1; Delta-aminolevulinate synthase 1
Protein Family:Alanine--tRNA ligase
UniProt Gene Name:ALAS1  
UniProt Entry Name:HEM1_HUMAN
Anti-ALAS1 Antibody (CAB6521)Western blot analysis of extracts of various cell lines, using ALAS1 antibody (CAB6521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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