Metabolism Antibodies 3
Anti-ALAD Antibody (CAB8398)
- SKU:
- CAB8398
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ALAD Antibody |
| Antibody SKU: | CAB8398 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, MCF7, 22Rv1, HL-60 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ALAD (NP_000022.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQPQ SVLH SGYF HPLL RAWQ TATT TLNA SNLI YPIF VTDV PDDI QPIT SLPG VARY GVKR LEEM LRPL VEEG LRCV LIFG VPSR VPKD ERGS AADS EESP AIEA IHLL RKTF PNLL VACD VCLC PYTS HGHC GLLS ENGA FRAE ESRQ RLAE VALA YAKA GCQV VAPS DMMD GRVE AIKE ALMA HGLG NRVS VMSY SAKF ASCF YGPF RDAA KSSP AFGD RRCY QLPP GARG LALR AVDR DVRE GADM LMVK PGMP YLDI VREV KDKH PDLP LAVY HVSG EFAM LWHG AQAG AFDL KAAV LEAM TAFR RAGA DIII TYYT PQLL QWLK EE |
| Gene ID: | 210 |
| Uniprot: | P13716 |
| Cellular Location: | |
| Calculated MW: | 36kDa/39kDa |
| Observed MW: | 35kDa |
| Synonyms: | ALAD, ALADH, PBGS |
| Background: | The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | ALAD: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Defects in ALAD are the cause of acute hepatic porphyria (AHEPP). A form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AHP is characterized by attacks of gastrointestinal disturbances, abdominal colic, paralysis, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. Belongs to the ALADH family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 4.2.1.24; Lyase Chromosomal Location of Human Ortholog: 9q32 Cellular Component: cytosol; extracellular region; nucleus Molecular Function:catalytic activity; identical protein binding; porphobilinogen synthase activity; zinc ion binding Biological Process: heme biosynthetic process; neutrophil degranulation; protein homooligomerization Disease: Porphyria, Acute Hepatic |
| NCBI Summary: | The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P13716 |
| NCBI GenInfo Identifier: | 122833 |
| NCBI Gene ID: | 210 |
| NCBI Accession: | P13716.1 |
| UniProt Secondary Accession: | P13716,Q16870, Q16871, Q9BVQ9, A8K375, B2R6F2, |
| UniProt Related Accession: | P13716 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | Delta-aminolevulinic acid dehydratase |
| NCBI Synonym Full Names: | aminolevulinate dehydratase |
| NCBI Official Symbol: | ALAD |
| NCBI Official Synonym Symbols: | PBGS; ALADH |
| NCBI Protein Information: | delta-aminolevulinic acid dehydratase |
| UniProt Protein Name: | Delta-aminolevulinic acid dehydratase |
| UniProt Synonym Protein Names: | Porphobilinogen synthase |
| Protein Family: | Aladin |
| UniProt Gene Name: | ALAD |
 | Western blot analysis of extracts of various cell lines, using ALAD antibody (CAB8398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using ALAD antibody (CAB8398) at dilution of 1:100 (40x lens). |
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