KO Validated Antibodies 1
Anti-AKT2 Antibody (CAB18019)[KO Validated]
- SKU:
- CAB18019
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- ICC
- Applications:
- IF
- Applications:
- IP
- Applications:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
Antibody Name: | Anti-AKT2 Antibody [KO Validated] |
Antibody SKU: | CAB18019 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC ICC IF IP ChIP |
Reactivity: | Human, Mouse, Rat, Monkey |
Host Species: | Rabbit |
Immunogen: | Recombinant protein of human AKT2 |
Application: | WB IHC ICC IF IP ChIP |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 ICC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50 ChIP 1:20 - 1:100 |
Reactivity: | Human, Mouse, Rat, Monkey |
Positive Samples: | 293T |
Immunogen: | Recombinant protein of human AKT2 |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Gene ID: | 208 |
Uniprot: | P31751 |
Cellular Location: | Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein |
Calculated MW: | 51kDa/55kDa |
Observed MW: | 60kDa |
Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. | |
Western blot analysis of extracts of various cell lines, using AKT2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |