Cell Biology Antibodies 2
Anti-AKAP7 Antibody (CAB11728)
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- SKU:
- CAB11728
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AKAP7 Antibody |
| Antibody SKU: | CAB11728 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human AKAP7 (NP_004833.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human AKAP7 (NP_004833.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGQL CCFP FSRD EGKI SEKN GGEP DDAE LVRL SKRL VENA VLKA VQQY LEET QNKN KPGE GSSV KTEA ADQN GNDN ENNR K |
| Gene ID: | 9465 |
| Uniprot: | O43687/Q9P0M2 |
| Cellular Location: | Apical cell membrane, Lateral cell membrane, Lipid-anchor, Lipid-anchor |
| Calculated MW: | 8kDa/11kDa/39kDa |
| Observed MW: | 14kDa |
| Synonyms: | AKAP7, AKAP15, AKAP18 |
| Background: | This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | AKAP7 iso1: Probably targets cAMP-dependent protein kinase (PKA) to the cellular membrane or cytoskeletal structures. The membrane- associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 6q23.2 Cellular Component: cytosol Molecular Function:protein binding; protein kinase A binding Biological Process: protein localization |
| NCBI Summary: | This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Apr 2011] |
| UniProt Code: | O43687 |
| NCBI GenInfo Identifier: | 25108878 |
| NCBI Gene ID: | 9465 |
| NCBI Accession: | O43687.4 |
| UniProt Secondary Accession: | O43687,Q5TBR9, Q5TBS0, Q9HCZ8, Q9P0G4, A8K2K6, |
| UniProt Related Accession: | Q9P0M2,O43687 |
| Molecular Weight: | 11kDa |
| NCBI Full Name: | A-kinase anchor protein 7 isoforms alpha and beta |
| NCBI Synonym Full Names: | A-kinase anchoring protein 7 |
| NCBI Official Symbol: | AKAP7 |
| NCBI Official Synonym Symbols: | AKAP15; AKAP18 |
| NCBI Protein Information: | A-kinase anchor protein 7 isoform gamma; A-kinase anchor protein 7 |
| UniProt Protein Name: | A-kinase anchor protein 7 isoforms alpha and beta |
| UniProt Synonym Protein Names: | A-kinase anchor protein 18 kDa; AKAP 18; Protein kinase A-anchoring protein 7 isoforms alpha/beta; PRKA7 isoforms alpha/beta |
| Protein Family: | A-kinase anchor protein |
| UniProt Gene Name: | AKAP7 |
 | Western blot analysis of extracts of various cell lines, using AKAP7 antibody (CAB11728) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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