Antibody Name:	Anti-AKAP5 Antibody
Antibody SKU:	CAB6520
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HepG2, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METT ISEI HVEN KDEK RSAE GSPG AERQ KEKA SMLC FKRR KKAA KALK PKAG SEAA DVAR KCPQ EAGA SDQP EPTR GAWA SLKR LVTR RKRS ESSK QQKP LEGE MQPA INAE DADL SKKK AKSR LKIP CIKF PRGP KRSN HSKI IEDS DCSI KVQE EAEI LDIQ TQTP LNDQ ATKA KSTQ DLSE GISR KDGD EVCE SNVS NSTT SGEK VISV ELGL DNGH SAIQ TGTL ILEE IETI KEKQ DVQP QQAS PLET SETD HQQP VLSD VPPL PA
Gene ID:	9495
Uniprot:	P24588
Cellular Location:	Lipid-anchor, Membrane
Calculated MW:	47kDa
Observed MW:	47kDa

Synonyms:

AKAP5, AKAP75, AKAP79, H21

Background:

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.

UniProt Protein Function:	AKAP5: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts. Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.
UniProt Protein Details:	Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 14q23.3 Cellular Component: cytosol; plasma membrane Molecular Function:adenylate cyclase binding; protein binding; protein kinase A binding Biological Process: positive regulation of cAMP biosynthetic process; synaptic transmission
NCBI Summary:	The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]
UniProt Code:	P24588
NCBI GenInfo Identifier:	281185503
NCBI Gene ID:	9495
NCBI Accession:	P24588.3
UniProt Secondary Accession:	P24588,A2RRB8,
UniProt Related Accession:	P24588
Molecular Weight:	47,088 Da
NCBI Full Name:	A-kinase anchor protein 5
NCBI Synonym Full Names:	A-kinase anchoring protein 5
NCBI Official Symbol:	AKAP5
NCBI Official Synonym Symbols:	H21; AKAP75; AKAP79
NCBI Protein Information:	A-kinase anchor protein 5
UniProt Protein Name:	A-kinase anchor protein 5
UniProt Synonym Protein Names:	A-kinase anchor protein 79 kDa; AKAP 79; H21; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Protein Family:	A-kinase anchor protein
UniProt Gene Name:	AKAP5
UniProt Entry Name:	AKAP5_HUMAN

	Western blot analysis of extracts of various cell lines, using AKAP5 Antibody (CAB6520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using AKAP5 antibody (CAB6520) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using AKAP5 antibody (CAB6520) at dilution of 1:100 (40x lens).