Cell Biology Antibodies 5

Anti-AKAP4 Antibody (CAB14813)

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SKU:
CAB14813
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AKAP4 Antibody
Antibody SKU:CAB14813
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 189-340 of human AKAP4 (NP_003877.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QSPS APPA KPPS TQRA VISP DGEC SIDD LSFY VNRL SSLV IQMA HKEI KEKL EGKS KCLH HSIC PSPG NKER ISPR TPAS KIAS EMAY EAVE LTAA EMRG TGEE SREG GQKS FLYS ELSN KSKS GDKQ MSQR ESKE FADS ISKG LMVY ANQV
Gene ID:8852
Uniprot:Q5JQC9
Cellular Location:Cell projection, cilium, flagellum
Calculated MW:93kDa/94kDa
Observed MW:82kDa
Synonyms:AKAP4, AKAP 82, AKAP-4, AKAP82, CT99, FSC1, HI, PRKA4, hAKAP82, p82
Background:The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
UniProt Protein Function:AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cancer Testis Antigen (CTA); Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex

Molecular Function:protein kinase A binding

Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility; signal transduction

NCBI Summary:The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:Q5JQC9
NCBI GenInfo Identifier:21493037
NCBI Gene ID:8852
NCBI Accession:NP_003877.2
UniProt Secondary Accession:Q5JQC9,O60904, O95246, Q5JQD1, Q5JQD2, Q5JQD3, A0AV85
UniProt Related Accession:Q5JQC9
Molecular Weight:94,477 Da
NCBI Full Name:A-kinase anchor protein 4 isoform 1
NCBI Synonym Full Names:A kinase (PRKA) anchor protein 4
NCBI Official Symbol:AKAP4  
NCBI Official Synonym Symbols:HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82  
NCBI Protein Information:A-kinase anchor protein 4; testis-specific gene HI; cancer/testis antigen 99; A-kinase anchor protein 82 kDa; major sperm fibrous sheath protein; protein kinase A anchoring protein 4; protein kinase A-anchoring protein 4
UniProt Protein Name:A-kinase anchor protein 4
UniProt Synonym Protein Names:A-kinase anchor protein 82 kDa; AKAP 82; hAKAP82; Major sperm fibrous sheath protein; HI; Protein kinase A-anchoring protein 4; PRKA4
Protein Family:A-kinase anchor protein
UniProt Gene Name:AKAP4  
UniProt Entry Name:AKAP4_HUMAN
Anti-AKAP4 Antibody (CAB14813)Western blot analysis of extracts of various cell lines, using AKAP4 antibody (CAB14813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3s.
Anti-AKAP4 Antibody (CAB14813)Immunofluorescence analysis of Rat testis using AKAP4 antibody (CAB14813) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-AKAP4 Antibody (CAB14813)Immunofluorescence analysis of Mouse testis using AKAP4 antibody (CAB14813) at dilution of 1:100. Blue: DAPI for nuclear staining.
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