Name: Anti-AKAP3 Antibody (CAB16484)
Brand: Cell Biology Antibodies 7
SKU: CAB16484
Availability: OutOfStock

Antibody Name:	Anti-AKAP3 Antibody
Antibody SKU:	CAB16484
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AKAP3 (NP_006413.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human AKAP3 (NP_006413.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSEK VDWL QSQN GVCK VDVY SPGD NQAQ DWKM DTST DPVR VLSW LRRD LEKS TAEF QDVR FKPG ESFG GETS NSGD PHKG FSVD YYNT TTKG TPER LHFE MTHK EIPC QGPR AQLG NGSS VDEV SFYA NRLT NLVI AMAR KEIN EKID GSEN KCVY QSLY MGNE PTPT KSLS KIAS ELVN ETVS ACSR NAAP DKAP GSGD RVSG SSQS PPNL KYKS TLKI KEST KERQ GPDD KPPS KKSF FYKE VFES RNGD YARE GGRF FPRE RKRF RGQE RPDD FTAS VSEG IMTY ANSV VSDM MVSI
Gene ID:	10566
Uniprot:	O75969
Cellular Location:	Cytoplasmic vesicle, acrosome, secretory vesicle
Calculated MW:	94kDa
Observed MW:	115kDa

Synonyms:

AKAP3, AKAP 110, AKAP110, CT82, FSP95, HEL159, PRKA3, SOB1

Background:

This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

UniProt Protein Function:	AKAP3: May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction. Belongs to the AKAP110 family.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: acrosome reaction; cell motility; single fertilization
NCBI Summary:	This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. [provided by RefSeq, May 2013]
UniProt Code:	O75969
NCBI GenInfo Identifier:	281185469
NCBI Gene ID:	10566
NCBI Accession:	O75969.2
UniProt Secondary Accession:	O75969,O75945, Q86X01, Q9UM61,
UniProt Related Accession:	O75969
Molecular Weight:	94,751 Da
NCBI Full Name:	A-kinase anchor protein 3
NCBI Synonym Full Names:	A-kinase anchoring protein 3
NCBI Official Symbol:	AKAP3
NCBI Official Synonym Symbols:	CT82; SOB1; FSP95; PRKA3; HEL159; AKAP110; AKAP 110
NCBI Protein Information:	A-kinase anchor protein 3
UniProt Protein Name:	A-kinase anchor protein 3
UniProt Synonym Protein Names:	A-kinase anchor protein 110 kDa; AKAP 110; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; FSP95; Fibrousheathin I; Fibrousheathin-1; Protein kinase A-anchoring protein 3; PRKA3; Sperm oocyte-binding protein
Protein Family:	A-kinase anchor protein
UniProt Gene Name:	AKAP3
UniProt Entry Name:	AKAP3_HUMAN

Western blot analysis of extracts of rat testis, using AKAP3 antibody (CAB16484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

AKAP3 Antibody (PACO03353)

AKAP3 Antibody (PACO07667)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)