Antibody Name:	Anti-AK1 Antibody
Antibody SKU:	CAB13460
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEK LKKT KIIF VVGG PGSG KGTQ CEKI VQKY GYTH LSTG DLLR SEVS SGSA RGKK LSEI MEKG QLVP LETV LDML RDAM VAKV NTSK GFLI DGYP REVQ QGEE FERR IGQP TLLL YVDA GPET MTQR LLKR GETS GRVD DNEE TIKK RLET YYKA TEPV IAFY EKRG IVRK VNAE GSVD SVFS QVCT HLDA LK
Gene ID:	203
Uniprot:	P00568
Cellular Location:	Cytoplasm
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

AK1, HTL-S-58j

Background:

This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.
UniProt Protein Details:	Protein type:EC 2.7.4.6; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.3 Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: cytoplasm; cytosol Molecular Function:adenylate kinase activity; nucleoside diphosphate kinase activity Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside diphosphate phosphorylation; nucleoside triphosphate biosynthetic process Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To
NCBI Summary:	This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:	P00568
NCBI GenInfo Identifier:	20178288
NCBI Gene ID:	203
NCBI Accession:	P00568.3
UniProt Secondary Accession:	P00568,Q9BVK9, Q9UQC7,
UniProt Related Accession:	P00568
Molecular Weight:	21,635 Da
NCBI Full Name:	Adenylate kinase isoenzyme 1
NCBI Synonym Full Names:	adenylate kinase 1
NCBI Official Symbol:	AK1
NCBI Official Synonym Symbols:	HTL-S-58j
NCBI Protein Information:	adenylate kinase isoenzyme 1
UniProt Protein Name:	Adenylate kinase isoenzyme 1
UniProt Synonym Protein Names:	ATP-AMP transphosphorylase 1
Protein Family:	Adenylate kinase
UniProt Gene Name:	AK1
UniProt Entry Name:	KAD1_HUMAN

	Western blot analysis of extracts of various cell lines, using AK1 antibody (CAB13460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody (CAB13460) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody (CAB13460) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody (CAB13460) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using AK1 antibody (CAB13460). Blue: DAPI for nuclear staining.