KO Validated Antibodies 1

Anti-AIF Antibody (CAB2568)[KO Validated]

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SKU:
CAB2568
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-AIF Antibody [KO Validated]
Antibody SKU:CAB2568
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW620, 22Rv1, H460, PC-3, U-937, Mouse liver, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FPEK GNMG KILP EYLS NWTM EKVR REGV KVMP NAIV QSVG VSSG KLLI KLKD GRKV ETDH IVAA VGLE PNVE LAKT GGLE IDSD FGGF RVNA ELQA RSNI WVAG DAAC FYDI KLGR RRVE HHDH AVVS GRLA GENM TGAA KPYW HQSM FWSD LGPD VGYE AIGL VDSS LPTV GVFA KATA QDNP KSAT EQSG TGIR SESE TESE ASEI TIPP STPA VPQA PVQG EDYG KGVI FYLR DKVV VGIV LWNI FNRM PIAR KIIK DGEQ HEDL NEVA KLFN IHED
Gene ID:9131
Uniprot:O95831
Cellular Location:Cytoplasm, Mitochondrion inner membrane, Mitochondrion intermembrane space, Nucleus, perinuclear region
Calculated MW:26kDa/28kDa/35kDa/66kDa
Observed MW:67kDa
Synonyms:AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, AIFM1
Background:This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.
UniProt Protein Function:PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; EC 1.-.-.-; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrion; nucleus

Molecular Function:NAD(P)H oxidase activity; oxidoreductase activity, acting on NADH or NADPH; protein binding

Biological Process: apoptosis; caspase activation; chromosome condensation; positive regulation of apoptosis

Disease: Deafness, X-linked 5

NCBI Summary:This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
UniProt Code:O95831
NCBI GenInfo Identifier:13431764
NCBI Gene ID:9131
NCBI Accession:O95831.1
UniProt Secondary Accession:O95831,Q1L6K4, Q1L6K6, Q2QKE4, Q5JUZ7, Q6I9X6, Q9Y3I3 Q9Y3I4, A4QPB4, B1ALN1, B2RB08, D3DTE9,
UniProt Related Accession:O95831
Molecular Weight:26,033 Da
NCBI Full Name:Apoptosis-inducing factor 1, mitochondrial
NCBI Synonym Full Names:apoptosis inducing factor mitochondria associated 1
NCBI Official Symbol:AIFM1  
NCBI Official Synonym Symbols:AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6  
NCBI Protein Information:apoptosis-inducing factor 1, mitochondrial
UniProt Protein Name:Apoptosis-inducing factor 1, mitochondrial
UniProt Synonym Protein Names:Programmed cell death protein 8
Protein Family:Putative apoptosis-inducing factor
UniProt Gene Name:AIFM1  
Anti-AIF Antibody [KO Validated] (CAB2568)Western blot analysis of extracts of various cell lines, using AIF antibody (CAB2568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-AIF Antibody [KO Validated] (CAB2568)Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (CAB2568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-AIF Antibody [KO Validated] (CAB2568)Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] AIF Rabbit pAb (CAB2568) at dilution of 1:50 (40x lens).
Anti-AIF Antibody [KO Validated] (CAB2568)Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] AIF Rabbit pAb (CAB2568) at dilution of 1:50 (40x lens).
Anti-AIF Antibody [KO Validated] (CAB2568)Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] AIF Rabbit pAb (CAB2568) at dilution of 1:50 (40x lens).
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