Cell Biology Antibodies 10

Anti-AHSP Antibody (CAB6465)

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SKU:
CAB6465
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AHSP Antibody
Antibody SKU:CAB6465
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human AHSP (NP_057717.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human AHSP (NP_057717.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALL KANK DLIS AGLK EFSV LLNQ QVFN DPLV SEED MVTV VEDW MNFY INYY RQQV TGEP QERD KALQ ELRQ ELNT LANP FLAK YRDF LKSH ELPS HPPP SS
Gene ID:51327
Uniprot:Q9NZD4
Cellular Location:Cytoplasm
Calculated MW:11kDa
Observed MW:12kDa
Synonyms:AHSP, EDRF, ERAF
Background:This gene encodes a molecular chaperone which binds specifically to free alpha-globin and is involved in hemoglobin assembly. The encoded protein binds to monomeric alpha-globin until it has been transferred to beta-globin to form a heterodimer, which in turn binds to another heterodimer to form the stable tetrameric hemoglobin. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:AHSP: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Belongs to the AHSP family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: hemoglobin complex

Molecular Function:hemoglobin binding; unfolded protein binding

Biological Process: protein folding; protein stabilization; hemoglobin metabolic process; hemopoiesis

UniProt Code:Q9NZD4
NCBI GenInfo Identifier:23813669
NCBI Gene ID:51327
NCBI Accession:Q9NZD4.1
UniProt Secondary Accession:Q9NZD4,Q8TD01,
UniProt Related Accession:Q9NZD4
Molecular Weight:102
NCBI Full Name:Alpha-hemoglobin-stabilizing protein
NCBI Synonym Full Names:alpha hemoglobin stabilizing protein
NCBI Official Symbol:AHSP  
NCBI Official Synonym Symbols:EDRF; ERAF  
NCBI Protein Information:alpha-hemoglobin-stabilizing protein; erythroid associated factor; erythroid-associated factor; alpha hemoglobin stabilising protein; erythroid differentiation-related factor; erythroid differentiation associated factor
UniProt Protein Name:Alpha-hemoglobin-stabilizing protein
UniProt Synonym Protein Names:Erythroid differentiation-related factor; Erythroid-associated factor
Protein Family:Alpha-hemoglobin-stabilizing protein
UniProt Gene Name:AHSP  
UniProt Entry Name:AHSP_HUMAN
Anti-AHSP Antibody (CAB6465)Western blot analysis of extracts of various cell lines, using AHSP antibody (CAB6465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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