Cell Biology Antibodies 6
Anti-AGK Mouse Monoclonal Antibody (CAB16230)
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- SKU:
- CAB16230
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Mouse
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AGK Mouse Monoclonal Antibody |
| Antibody SKU: | CAB16230 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Mouse |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human AGK (NP_060708.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, 293T, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human AGK (NP_060708.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTVF FKTL RNHW KKTT AGLC LLTW GGHW LYGK HCDN LLRR AACQ EAQV FGNQ LIPP NAQV KKAT VFLN PAAC KGKA RTLF EKNA APIL HLSG MDVT IVKT DYEG QAKK LLEL MENT DVII VAGG DGTL QEVV TGVL RRTD EATF SKIP IGFI PLGE TSSL SHTL FAES GNKV QHIT DATL AIVK GETV PLDV LQIK GEKE QPVF AMTG LRWG SFRD AGVK VSKY WYLG PLKI KAAH FFST LKEW PQTH QASI SYTG PTER PPNE PEET PVQR PSLY RRIL RRLA SYWA QPQD ALSQ EVSP EVWK DVQL STIE LSIT TRNN QLDP TSKE DFLN ICIE PDTI SKGD FITI GSRK VRNP KLHV EGTE CLQA SQCT LLIP EGAG GSFS IDSE EYEA MPVE VKLL PRKL QFFC DPRK REQM LTSP TQ |
| Gene ID: | 55750 |
| Uniprot: | Q53H12 |
| Cellular Location: | Mitochondrion membrane |
| Calculated MW: | 7kDa/47kDa |
| Observed MW: | 47kDa |
| Synonyms: | AGK, CATC5, CTRCT38, MTDPS10, MULK |
| Background: | The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. |
| UniProt Protein Function: | AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; EC 2.7.1.94 Chromosomal Location of Human Ortholog: 7q34 Cellular Component: mitochondrion; intracellular membrane-bound organelle; mitochondrial membrane Molecular Function:acylglycerol kinase activity; ceramide kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity Biological Process: protein kinase C activation; glycerolipid metabolic process; ceramide biosynthetic process; lipid phosphorylation Disease: Cataract 38; Sengers Syndrome |
| NCBI Summary: | The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q53H12 |
| NCBI GenInfo Identifier: | 8922701 |
| NCBI Gene ID: | 55750 |
| NCBI Accession: | NP_060708.1 |
| UniProt Secondary Accession: | Q53H12,Q75KN1, Q96GC3, Q9NP48, |
| UniProt Related Accession: | Q53H12 |
| Molecular Weight: | 47,137 Da |
| NCBI Full Name: | acylglycerol kinase, mitochondrial |
| NCBI Synonym Full Names: | acylglycerol kinase |
| NCBI Official Symbol: | AGK |
| NCBI Official Synonym Symbols: | MULK; CATC5; CTRCT38; MTDPS10 |
| NCBI Protein Information: | acylglycerol kinase, mitochondrial; hAGK; hsMuLK; multi-substrate lipid kinase; multiple substrate lipid kinase |
| UniProt Protein Name: | Acylglycerol kinase, mitochondrial |
| UniProt Synonym Protein Names: | Multiple substrate lipid kinase |
| Protein Family: | Acylglycerol kinase |
| UniProt Gene Name: | AGK |
| UniProt Entry Name: | AGK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AGK antibody (CAB16230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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