Cell Biology Antibodies 12

Anti-AGK Antibody (CAB9976)

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SKU:
CAB9976
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AGK Antibody
Antibody SKU:CAB9976
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK (NP_060708.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:293T, SH-SY5Y, HepG2, HeLa, 22Rv1, BT-474, K-562, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK (NP_060708.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KAAH FFST LKEW PQTH QASI SYTG PTER PPNE PEET PVQR PSLY RRIL RRLA SYWA QPQD ALSQ EVSP EVWK DVQL STIE LSIT TRNN QLDP TSKE DFLN ICIE PDTI SKGD FITI GSRK VRNP KLHV EGTE CLQA SQCT LLIP EGAG GSFS IDSE EYEA MPVE VKLL PRKL QFFC DPRK REQM LTSP TQ
Gene ID:55750
Uniprot:Q53H12
Cellular Location:Mitochondrion membrane
Calculated MW:7kDa/47kDa
Observed MW:47kDa
Synonyms:AGK, CATC5, CTRCT38, MTDPS10, MULK
Background:The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10.
UniProt Protein Function:AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; EC 2.7.1.94

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: mitochondrion; intracellular membrane-bound organelle; mitochondrial membrane

Molecular Function:acylglycerol kinase activity; ceramide kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: protein kinase C activation; glycerolipid metabolic process; ceramide biosynthetic process; lipid phosphorylation

Disease: Cataract 38; Sengers Syndrome

NCBI Summary:The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]
UniProt Code:Q53H12
NCBI GenInfo Identifier:8922701
NCBI Gene ID:55750
NCBI Accession:NP_060708.1
UniProt Secondary Accession:Q53H12,Q75KN1, Q96GC3, Q9NP48,
UniProt Related Accession:Q53H12
Molecular Weight:47,137 Da
NCBI Full Name:acylglycerol kinase, mitochondrial
NCBI Synonym Full Names:acylglycerol kinase
NCBI Official Symbol:AGK  
NCBI Official Synonym Symbols:MULK; CATC5; CTRCT38; MTDPS10  
NCBI Protein Information:acylglycerol kinase, mitochondrial; hAGK; hsMuLK; multi-substrate lipid kinase; multiple substrate lipid kinase
UniProt Protein Name:Acylglycerol kinase, mitochondrial
UniProt Synonym Protein Names:Multiple substrate lipid kinase
Protein Family:Acylglycerol kinase
UniProt Gene Name:AGK  
UniProt Entry Name:AGK_HUMAN
Anti-AGK Polyclonal Antibody (CAB9976)Western blot analysis of extracts of various cell lines, using AGK antibody (CAB9976) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
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