Name: Anti-AGAP2 Antibody (CAB2759)
Brand: Cell Biology Antibodies 8
SKU: CAB2759
Availability: OutOfStock

Antibody Name:	Anti-AGAP2 Antibody
Antibody SKU:	CAB2759
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 627-836 of human AGAP2 (NP_055585.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, A-549, Raji, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 627-836 of human AGAP2 (NP_055585.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VRSL DLDD WPRE LTLV LTAI GNDT ANRV WESD TRGR AKPS RDSS REER ESWI RAKY EQLL FLAP LSTS EEPL GRQL WAAV QAQD VATV LLLL AHAR HGPL DTSV EDPQ LRSP LHLA AELA HVVI TQLL LWYG ADVA ARDA QGRT ALFY ARQA GSQL CADI LLQH GCPG EGGS AATT PSAA TTPS ITAT PSPR RRSS AASV GRAD APVA LV
Gene ID:	116986
Uniprot:	Q99490
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	90kDa/124kDa
Observed MW:	110kDa

Synonyms:	AGAP2, CENTG1, GGAP2, PIKE
Background:	The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	CENTG1: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins (GAPs). A GAP for ARF1 and ARF5. Two alternatively-spliced isoforms have been described. Isoform 1 is brain-specific, and isoform 2 is ubiquitously expressed, with highest levels in brain and heart. Isoform 1 participates to the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. The PH domains of isoforms 1 and 2 bind phospholipids, but they differentially regulate subcellular location. The PH domain of isoform 1 directs the protein to the nucleus, but the PH domain of isoform 2 directs it to the cytosol.
UniProt Protein Details:	Protein type:GAPs; Motility/polarity/chemotaxis; Tumor suppressor; GAPs, ARF Chromosomal Location of Human Ortholog: 12q14.1 Cellular Component: membrane; mitochondrion; nucleolus; nucleus Molecular Function:protein binding Biological Process: negative regulation of neuron apoptosis; negative regulation of protein catabolic process
NCBI Summary:	The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q99490
NCBI GenInfo Identifier:	97535883
NCBI Gene ID:	116986
NCBI Accession:	Q99490.2
UniProt Secondary Accession:	Q99490,O00578, Q548E0, Q8IWU3, A8K9F7,
UniProt Related Accession:	Q99490
Molecular Weight:	90,537 Da
NCBI Full Name:	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
NCBI Synonym Full Names:	ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
NCBI Official Symbol:	AGAP2
NCBI Official Synonym Symbols:	PIKE; GGAP2; CENTG1
NCBI Protein Information:	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
UniProt Protein Name:	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
UniProt Synonym Protein Names:	Centaurin-gamma-1; Cnt-g1; GTP-binding and GTPase-activating protein 2; GGAP2; Phosphatidylinositol 3-kinase enhancer; PIKE
Protein Family:	Arf-GAP with GTPase, ANK repeat and PH domain-containing protein
UniProt Gene Name:	AGAP2
UniProt Entry Name:	AGAP2_HUMAN

Western blot analysis of extracts of various cell lines, using AGAP2 antibody (CAB2759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

AGAP2 Antibody (PACO07638)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)