Cell Biology Antibodies 2
Anti-AFP Mouse Monoclonal Antibody (CAB11865)
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- SKU:
- CAB11865
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-AFP Mouse Monoclonal Antibody |
| Antibody SKU: | CAB11865 |
| Antibody Size: | 20uL, 50uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Mouse |
| Immunogen: | Recombinant protein of human AFP |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, human plasma, SH-SY5Y, mouse plasma, mouse liver, mouse kidney, rat plasma, rat liver |
| Immunogen: | Recombinant protein of human AFP |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 174 |
| Uniprot: | P02771 |
| Cellular Location: | Secreted |
| Calculated MW: | 68kDa |
| Observed MW: | 69kDa |
| Synonyms: | AFPD, FETA, HPAFP, AFP |
| Background: | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
| UniProt Protein Function: | AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: extracellular space; cytoplasm Molecular Function:metal ion binding Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of |
| NCBI Summary: | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P02771 |
| NCBI GenInfo Identifier: | 120042 |
| NCBI Gene ID: | 174 |
| NCBI Accession: | P02771.1 |
| UniProt Secondary Accession: | P02771,B2RBU3, |
| UniProt Related Accession: | P02771 |
| Molecular Weight: | 609 |
| NCBI Full Name: | Alpha-fetoprotein |
| NCBI Synonym Full Names: | alpha-fetoprotein |
| NCBI Official Symbol: | AFP |
| NCBI Official Synonym Symbols: | AFPD; FETA; HPAFP |
| NCBI Protein Information: | alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein |
| UniProt Protein Name: | Alpha-fetoprotein |
| UniProt Synonym Protein Names: | Alpha-1-fetoprotein; Alpha-fetoglobulin |
| Protein Family: | AFP |
| UniProt Gene Name: | AFP |
| UniProt Entry Name: | FETA_HUMAN |
 | Western blot analysis of extracts of various cells, using AFP antibody (CAB11865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using AFP antibody (CAB11865) at dilution of 1:12.5 (40x lens). |
 | Immunofluorescence analysis of human liver cancer using AFP antibody (CAB11865) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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