Cell Biology Antibodies 2

Anti-AFP Mouse Monoclonal Antibody (CAB11865)

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SKU:
CAB11865
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AFP Mouse Monoclonal Antibody
Antibody SKU:CAB11865
Antibody Size:20uL, 50uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Mouse
Immunogen:Recombinant protein of human AFP
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, human plasma, SH-SY5Y, mouse plasma, mouse liver, mouse kidney, rat plasma, rat liver
Immunogen:Recombinant protein of human AFP
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:174
Uniprot:P02771
Cellular Location:Secreted
Calculated MW:68kDa
Observed MW:69kDa
Synonyms:AFPD, FETA, HPAFP, AFP
Background:This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
UniProt Protein Function:AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function:metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

NCBI Summary:This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
UniProt Code:P02771
NCBI GenInfo Identifier:120042
NCBI Gene ID:174
NCBI Accession:P02771.1
UniProt Secondary Accession:P02771,B2RBU3,
UniProt Related Accession:P02771
Molecular Weight:609
NCBI Full Name:Alpha-fetoprotein
NCBI Synonym Full Names:alpha-fetoprotein
NCBI Official Symbol:AFP  
NCBI Official Synonym Symbols:AFPD; FETA; HPAFP  
NCBI Protein Information:alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name:Alpha-fetoprotein
UniProt Synonym Protein Names:Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family:AFP
UniProt Gene Name:AFP  
UniProt Entry Name:FETA_HUMAN
Anti-AFP Mouse Monoclonal Antibody (CAB11865)Western blot analysis of extracts of various cells, using AFP antibody (CAB11865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-AFP Mouse Monoclonal Antibody (CAB11865)Immunohistochemistry of paraffin-embedded human liver cancer using AFP antibody (CAB11865) at dilution of 1:12.5 (40x lens).
Anti-AFP Mouse Monoclonal Antibody (CAB11865)Immunofluorescence analysis of human liver cancer using AFP antibody (CAB11865) at dilution of 1:100. Blue: DAPI for nuclear staining.
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