Cell Biology Antibodies 7

Anti-AFP Antibody (CAB16750)

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SKU:
CAB16750
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AFP Antibody
Antibody SKU:CAB16750
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human AFP.
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, Mouse liver, Mouse kidney, Rat plasma, Rat liver
Immunogen:Recombinant protein of human AFP.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:174
Uniprot:P02771
Cellular Location:
Calculated MW:68kDa
Observed MW:75kDa
Synonyms:AFPD, FETA, HPAFP, AFP
Background:This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
UniProt Protein Function:AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function:metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

NCBI Summary:This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
UniProt Code:P02771
NCBI GenInfo Identifier:120042
NCBI Gene ID:174
NCBI Accession:P02771.1
UniProt Secondary Accession:P02771,B2RBU3,
UniProt Related Accession:P02771
Molecular Weight:609
NCBI Full Name:Alpha-fetoprotein
NCBI Synonym Full Names:alpha-fetoprotein
NCBI Official Symbol:AFP  
NCBI Official Synonym Symbols:AFPD; FETA; HPAFP  
NCBI Protein Information:alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name:Alpha-fetoprotein
UniProt Synonym Protein Names:Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family:AFP
UniProt Gene Name:AFP  
UniProt Entry Name:FETA_HUMAN
Anti-AFP Antibody (CAB16750)Western blot analysis of extracts of various cell lines, using AFP antibody (CAB16750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-AFP Antibody (CAB16750)Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control) (upper right) using AFP Rabbit pAb (red, A16750) at dilution of 1:100. Blue: DAPI for nuclear staining.
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